Helicobacter Species: Methods and Protocols

Book Overview

1. Introduction

JeanMarie Houghton

2. Helicobacter pylori: An Overview

Jennifer M. Noto and Richard M. Peek, Jr.

3. Perspectives on Methodology for in vitro Culture of Helicobacter pylori

Timothy L. Cover

4. Successful Culture Techniques for Helicobacter Species: General Culture Techniques for Helicobacter pylori

Jeannette M. Whitmire and D. Scott Merrell

5. Successful Culture Techniques for Helicobacter Species: Establishing H. pylori Cultures From Infected Rodents

Jeannette M. Whitmire and D. Scott Merrell

6. Successful Culture Techniques for Helicobacter Species: Verification of Helicobacter Identity Using 16S rRNA Gene Sequence Analysis

Jeannette M. Whitmire and D. Scott Merrell

7. The Helicobacter pylori cag Pathogenicity Island

Jennifer M. Noto and Richard M. Peek, Jr.

8. Genetic Manipulation of a Naturally Competent Bacterium, Helicobacter pylori

Jennifer M. Noto and Richard M. Peek, Jr.

9. A Method for Short-term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori

Marina Leite and Ceu Figueiredo 10. Cell Culture Based Assays to Test for Bacterial Adherence and Internalization

Deepa Raju, David Rizzuti and Nicola L. Jones

11. Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence

Deepa Raju, David Rizzuti, Nicola L. Jones

12. Rodent Models of Helicobacter Infection, Inflammation and Disease

Songhua Zhang and Steven F. Moss

13. Bacterial Culture and Inoculation of Mice (Simple Infection)

Brian M. Gray and Kathryn A. Eaton

14. Adoptive Transfer of Splenocytes to Immunocompromised Mice

Brian M. Gray and Kathryn A. Eaton

15. Isolation of Gastric Lamina Propria Leukocytes (LPL)

Brian M. Gray and Kathryn A. Eaton

16. Delayed-type Hypersensitivity Determination

Brian M. Gray and Kathryn A. Eaton

17. Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression

Brian M. Gray and Kathryn A. Eaton

18. Animal Models of Helicobacter Induced Disease - Methods to Successfully Infect the Mouse

Nancy S. Taylor and James G. Fox

19. Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice

Mark T Whary, Zhongming Ge and James G Fox

20. Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes

Richard L. Ferrero, John E. Wilson, and Phil Sutton

21. Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections

Arlin B. Rogers

22. Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models

Arlin B. Rogers

23. Innate Immune Responses to Helicobacter pylori Infection- An Overview

Milan K. Patel, Melanie I. Trombly and Evelyn A. Kurt-Jones

24. Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection

Milan K. Patel, Glennice N. Bowen, Anna M. Cerny and Evelyn A. Kurt-Jones

25. Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase Detection

Michael Craig, Michael Schumacher and Yana Zavros

26. In vivo Measurement of Helicobacter pylori Infection

Marjan Mohammadi, Samaneh Saberi Kashani, Yeganeh Talebkhan Garoosi, Sahar Jahangiri Tazehkand

10. Cell Culture Based Assays to Test for Bacterial Adherence and Internalization

Deepa Raju, David Rizzuti and Nicola L. Jones

11.

• Format: Paperback
• Language: English
• ISBN: 1493959077
• ISBN13: 9781493959075
• Release Date: August 2016
• Publisher: Humana
• Length: 258 Pages
• Weight: 1.04 lbs.
• Dimensions: 0.6" x 7.0" x 10.0"