Proteomics in Systems Biology: Methods and Protocols

1. Review of the Real and Sometimes Hidden Costs in Proteomics Experimental Workflows

Aicha Asma Houfani and Leonard James Foster

2. High-Throughput Mass Spectrometry-Based Proteomics with dia-PASEF

Patricia Skowronek and Florian Meier

3. Isolation of Detergent Insoluble Proteins from Mouse Brain Tissue for Quantitative Analysis Using Data Independent Acquisition (DIA)

Cristen Molzahn, Lorenz Nierves, Philipp F. Lange, and Thibault Mayor

4. Rodent Lung Tissue Sample Preparation and Processing for Shotgun Proteomics

Hadeesha Piyadasa, Ying Lao, Oleg Krokhin, and Neeloffer Mookherjee

5. Protein Purification and Digestion Methods for Bacterial Proteomic Analyses

Nicole Hansmeier, Samrachana Sharma, and Tzu-Chiao Chao

6. Mapping Cell Surface Proteolysis with Plasma Membrane-Targeted Subtiligase

Aspasia A. Amiridis and Amy M. Weeks

7. N-Terminomics/TAILS of Tissue and Liquid Biopsies

Anthonia Anowai, Sameeksha Chopra, Barbara Mainoli, Daniel Young, and Antoine Dufour

8. HUNTER: Sensitive Automated Characterization of Proteolytic Systems by N Termini Enrichment from Microscale Specimen

Anuli C. Uzozie, Janice Tsui, and Philipp F. Lange

9. Phosphoproteomics and Organelle Proteomics in Pancreatic Islets

?zum Sehnaz Caliskan, Giorgia Massacci, Natalie Krahmer, and Francesca Sacco

10. Phosphoproteomic Sample Preparation for Global Phosphorylation Profiling of a Fungal Pathogen

Brianna Ball, Jonathan Krieger, and Jennifer Geddes-McAlister

11. Glycopeptide-Centric Approaches for the Characterization of Microbial Glycoproteomes

Nichollas E. Scott

12. Integrated Network Discovery Using Multi-Proteomic Data

Rafe Helwer and Vincent C. Chen

13. Targeted Cross-Linking Mass Spectrometry on Single-Step Affinity Purified Molecular Complexes in the Yeast Saccharomyces cerevisiae

Christian Trahan and Marlene Oeffinger

14. A Crosslinking - Mass Spectrometry Protocol for the Structural Analysis of Microtubule-Associated Proteins

Atefeh Rafiei and David C. Schriemer

15. Comprehensive Interactome Mapping of Nuclear Receptors Using Proximity Biotinylation

Lynda Agbo, Sophie Anne Blanchet, Pata-Eting Kougnassoukou Tchara, Am?lie Fradet-Turcotte, and Jean-Philippe Lambert

16. Mining Proteomics Datasets to Uncover Functional Pseudogenes

Anna Meller and Fran?ois-Michel Boisvert

17.

• Format: Hardcover
• Language: English
• ISBN: 1071621238
• ISBN13: 9781071621233
• Release Date: May 2022
• Publisher: Humana
• Length: 370 Pages
• Weight: 1.95 lbs.
• Dimensions: 0.9" x 7.0" x 10.0"