Handbook of Cryo-Preparation Methods for Electron Microscopy

Documenting the exponential growth in cryo-electron microscopy, the resulting advances, new hypotheses, and heightened understanding of the ultrastructure of living and inert matter, this book employes an accessible, step-by-step format to present the most sophisticated methods used in research laboratories today. It describes the basic principles of cryo-fixation, cryo-ultramicrotomy, embedding at low temperatures, and freeze-fracture. It explores applications in electron crystallography, 2D/3D structure analysis, cryo-electron tomography, and techniques for protein localization. Each chapter covers a specific method, including detailed protocols, summary tables, and hundreds of illustrations and diagrams.

• Format: Hardcover
• Language: English
• ISBN: 0849372275
• ISBN13: 9780849372278
• Release Date: October 2008
• Publisher: CRC Press
• Length: 706 Pages
• Weight: 3.00 lbs.
• Dimensions: 1.4" x 7.0" x 10.0"