Signal Transduction Protocols

Book Overview

Part I. Overviews

1. Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling

Louis M. Luttrell and Terry P. Kenakin

2. Imaging-based Approaches to Understanding G Protein-Coupled Receptor Signaling Complexes

Darlaine P?trin and Terence E. H?bert

3. Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis

John K. Westwick and Jane E. Lamerdin

4. RGS-insensitive Ga Subunits: Probes of G Subtype-selective Signaling and Physiological Functions of RGS Proteins

Kuljeet Kaur, Jason M. Kehrl, Raelene A. Charbeneau, and Richard R. Neubig

5. Bioinformatic Approaches to Metabolic Pathways Analysis

Stuart Maudsley, Wayne Chadwick, Liyun Wang, Yu Zhou, Bronwen Martin, and Sung Park

Part II. Receptor - Ligand Interactions

6. Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET

Jean-Pierre Vilardaga

7. Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signaling Complexes

Nicolas Audet and Graciela Pi?eyro

Part III. Receptor - Receptor Interactions

8. Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High Density Lipoprotein Particles (rHDL)

Gisselle A. V?lez-Ruiz and Roger K. Sunahara

9. Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Hetero-Dimerization

Lamia Achour, Maud Kamal, Ralf Jockers, and Stefano Marullo

10. Cell-Surface Protein-Protein Interaction Analysis with Time-Resolved FRET and Snap-tag Technologies: Application to G Protein-Coupled Receptor Oligomerization

La?titia Comps-Agrar, Damien Maurel, Philippe Rondard, Jean-Philippe Pin, Trinquet Eric, and Laurent Pr?zeau

11. Analysis of GPCR/Ion Channel Interactions

Christophe Altier and Gerald W. Zamponi

Part IV. Receptor - Effector Coupling

12. Multicolor BiFC Analysis of G Protein  Complex Formation and Localization

Thomas R. Hynes, Evan A. Yost, Stacy M. Yost, and Catherine H. Berlot

13. Real-Time BRET Assays to Measure G Protein/Effector Interactions

Darlaine P?trin, M?lanie Robitaille, and Terence E. H?bert

14. Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP

Brock F. Binkowski, Frank Fan, and Keith V. Wood

15. Simultaneous Real Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters

Lianne B. Dale and Stephen S. G. Ferguson

Part V. Spatial Control of Signal Transduction

16. Using FRET-based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity

Charlene Depry and Jin Zhang

17. Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells

Lisa L. Gallegos and Alexandra C. Newton

18. Visualizing Receptor Endocytosis and Trafficking

Ali Salahpour and Larry S. Barak

19. Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM

Guillermo A. Yudowski and Mark von Zastrow

20. Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy

Sudha K. Shenoy

Part VI. Protein - Protein Interactions

21. Detection and Characterization of Receptor Interactions with PDZ Domains

Stefanie L. Ritter and Randy A. Hall

22. Tandem Affinity Purification and Identification of Heterotrimeric G-Protein Associated Proteins

Syed M.

• Format: Paperback
• Language: English
• ISBN: 149396108X
• ISBN13: 9781493961085
• Release Date: August 2016
• Publisher: Humana
• Length: 430 Pages
• Weight: 1.69 lbs.
• Dimensions: 0.9" x 7.0" x 10.0"