Regulatory T Cells: Methods and Protocols

Book Overview

Part I: Introduction 1. Regulatory T Cells: History and Perspective Shimon Sakaguchi Part II: In vitro 2. In vitro Treg Suppression Assays Lauren W. Collison and Dario A.A. Vignali 3. Generation of T Cell Hybridomas from Naturally-Occurring FoxP3+ Regulatory T Cells Nagendra Singh, Rafal Pacholczyk, Makio Iwashima, and Leszek Ignatowicz 4. In vitro and In vivo Analyses of Regulatory T Cell Suppression of CD8+ T Cells Kim J. Hasenkrug and Lara M. Myers 5. Flow Cytometric Profiling of Mature and Developing Regulatory T Cells in the Thymus Donald M. Simons and Andrew J. Caton 6. ChIP-on-Chip for FoxP3 Ye Zheng 7. Live Imaging of Dendritic Cell-Treg Cell Interactions Milka Sarris and Alexander G. Betz Part III: In vivo 8. Genetic Tools for Analysis of FoxP3+ Regulatory T Cells In vivo Nadia M. Jeremiah and Adrian Liston 9. In vivo Treg Suppression Assays Creg J. Workman, Lauren W. Collison, Maria Bettini, Meenu R. Pillai, Jerold E. Rehg, and Dario A.A. Vignali 10. In vivo Depletion of FoxP3+ Tregs Using the DEREG Mouse Model Katharina Lahl and Tim Sparwasser 11. Antigen-Specific Induction of Regulatory T Cells In vivo and In vitro Carolin Daniel, Hidde Ploegh, and Harald von Boehmer 12. In vitro Expansion of Alloantigen-Specific Regulatory T Cells and Their Use in Prevention of Allograft Rejection Cl?mence Nouz?, Lise Pasquet, and Joost P.M. van Meerwijk Part IV: Human 13. Analysis of Human FoxP3+ Treg Cells Phenotype and Function Eva d'Hennezel and Ciriaco A. Piccirillo 14. Depletion of Human Regulatory T Cells Amy C. Hobeika, Michael A. Morse, Takuya Osada, Sharon Peplinski, H. Kim Lyerly, and Timothy M. Clay 15. Assessment of Suppressive Capacity by Human Regulatory T Cells Using a Reproducible, Bi-Directional CFSE-Based In vitro Assay Anya Schneider and Jane H. Buckner 16. Measurement of Proliferation and Disappearance of Regulatory T Cells in Human Studies Using Deuterium-Labeled Glucose Milica Vukmanovic-Stejic, Yan Zhang, Arne N. Akbar, and Derek C. Macallan 17. Flow Cytometric Detection of Human Regulatory T Cells Barbara Fazekas de St. Groth, Erhua Zhu, Suzanne Asad, and Loretta Lee

• Format: Paperback
• Language: English
• ISBN: 1493961586
• ISBN13: 9781493961580
• Release Date: August 2016
• Publisher: Humana
• Length: 282 Pages
• Weight: 1.14 lbs.
• Dimensions: 0.6" x 7.0" x 10.0"