Phytoplasma: Methods and Protocols

Book Overview

1. The Phytoplasmas - An Introduction

Matt Dickinson, Melanie Tuffen, and Jennifer Hodgetts

2. Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus

Jennifer Hodgetts, David Crossley, and Matt Dickinson

3. Micropropagation and Maintenance of Phytoplasmas in Tissue Culture

Assunta Bertaccini, Samanta Paltrinieri, Marta Martini, Mara Tedeschi, and Nicoletta Contaldo

4. Dodder Transmission of Phytoplasmas

Jaroslava Přibylov? and Josef Spak

5. Insect Maintenance and Transmission

Heather Kingdom

6. Capturing Insect Vectors of Phytoplasmas

Phyllis Weintraub and J?rgen Gross

7. Insect Vector Transmission Assays

Domenico Bosco and Rosemarie Tedeschi

8. Molecular Identification of Phytoplasma Vector Species

Sabrina Bertin and Domenico Bosco

9. Dienes' Staining and Light Microscopy for Phytoplasma Visualization

Rita Musetti

10. DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas

Nancy S. Andrade and Nolberto S. Arismendi

11. Visualisation of Phytoplasmas using Electron Microscopy

B. Jean Devonshire

12. Automated DNA Extraction for Large Numbers of Plant Samples

Natasa Mehle, Petra Nikolic, Matevz Rupar, Jana Boben, Maja Ravnikar, and Marina Dermastia

13. DNA Extraction from Arborescent Monocots and How To Deal With Other Challenging Hosts

Nigel A. Harrison, Robert E. Davis, and Ericka E. Helmick

14. Nested PCR and RFLP Analysis Based on the 16S rRNA Gene

Bojan Duduk, Samantha Paltrinieri, Ing Ming Lee, and Assunta Bertaccini

15. PCR and RFLP Analyses Based on the Ribosomal Protein Operon

Marta Martini and Ing-Ming Lee

16. Tuf and secY PCR Amplification and Genotyping of Phytoplasmas

Xavier Foissac, Jean-Luc Danet, Sylvie Malembic-Maher, Pascal Salar, Dana Saf?řov?, Pavla V?lov?, and Milan Navr?til

17. PCR Analysis of Phytoplasmas Based on the secA Gene

Matt Dickinson and Jennifer Hodgetts

18. Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains

Martina Seruga Music and Dijana Skoric

19. Microarrays for Universal Detection and Identification of Phytoplasmas

Mogens Nicolaisen, Henriette Nyskjold, and Assunta Bertaccine

20. T-RFLP for Detection and Identification of Phytoplasmas in Plants

Jennifer Hodgetts and Matt Dickinson

21. Real-Time PCR for Universal Phytoplasma Detection and Quantification

Nynne Meyn Christensen, Henriette Nyskjold, and Mogens Nicolaisen

22. A Real-Time PCR Detection System for the Bois Noir and Flavescence Dor?e Phytoplasmas and Quantification of the Target DNA

Natasa Mehle, Nina Prezelj, Matjaz Hren, Jana Boben, Kristina Gruden, Maja Ravnikar, and Marina Dermastia

23. Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group

Natasa Mehle, Petra Nikolic, Kristina Gruden, Maja Ravnikar, and Marina Dermastia

24. Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions

Paola Margaria and Sabrina Palmano

25. In-Field Diagnostics Using Loop-Mediated Isothermal Amplification (LAMP)

Jenny Tomlinson

26. DNA Bar-Coding

• Format: Hardcover
• Language: English
• ISBN: 1627030883
• ISBN13: 9781627030885
• Release Date: September 2012
• Publisher: Humana
• Length: 421 Pages
• Weight: 2.10 lbs.
• Dimensions: 1.1" x 7.0" x 10.3"