Optical Fluorescence Microscopy: From the Spectral to the Nano Dimension

By Alberto Diaspro

Book Overview

Fundamentals of Optical Microscopy.- The White Confocal - Continuous Spectral Tuning in Excitation and Emission.- SHG/THG (Second/Third Harmonic Generation) Microscopy.- Role of Scattering and Nonlinear Effects in the Illumination and the Photobleaching Distribution Profiles.- New Analytical Tools for Evaluation of Spherical Aberration in Optical Microscopy.- Improving Image Formation by Pushing the Signal to Noise Ratio.- Site-specific Labeling of Proteins in Living Cells Using Synthetic Fluorescent Dyes.- Imaging Molecular Physiology in Cells Using FRET-based Fluorescent Nanosensors.- Measuring Molecular Dynamics by FRAP, FCS and SPT.- In vitro - in-vivo Fluctuation Spectroscopies.- Interference X-ray Diffraction from Single Muscle Cells Reveals the Molecular Basis of Muscle Braking.- Low Concentration Protein Detection Using Novel SERS Devices.- Near Infrared 3-Dimensional Nonlinear Optical Monitoring of Stem Cell Differentiation.- A Correlative Microscopy: a Combination of Light and Electron Microscopy.

• Format: Paperback
• Language: English
• ISBN: 3642422810
• ISBN13: 9783642422812
• Release Date: November 2014
• Publisher: Springer
• Length: 244 Pages
• Weight: 0.81 lbs.
• Dimensions: 0.5" x 6.1" x 9.2"