Microrna Protocols

The MicroRNA.- Analysis of MicroRNA Length Variety Generated by Recombinant Human Dicer.- A User-Friendly Computational Workflow for the Analysis of miRNA Deep Sequencing Data.- The RNA Gene Information: Retroelement-MicroRNA Entangling as the RNA Quantum Code.- Setting Up an Intronic miRNA Database.- Identify Intronic MicroRNA with Bioinformatics.- Experimental miRNA Target Validation.- Enrichment Analysis of miRNA Targets.- MicroRNA Expression Profiling During Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells.- Evaluating the MicroRNA Targeting Sites by Luciferase Reporter Gene Assay.- Expression Pattern Analysis of MicroRNAs in Caenorhabditis elegans.- Use of Viral Systems to Study miRNA-Mediated Regulation of Gene Expression in Human Cells.- Cloning, Expression, and Functional Analysis of Genomic miRNA Using Retroviral System in Cancer Cells.- Preparing Synaptoneurosomes from Adult Mouse Forebrain.- Isolation of Total RNA and Detection Procedures for miRNA Present in Bovine Cultured Adipocytes and Adipose Tissues.- miRNA and shRNA Expression Vectors Based on mRNA and miRNA Processing.- Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs.- Vector-Free Methods for Manipulating miRNA Activity In Vitro and In Vivo.- MicroRNA Expression Profiling of Human Induced Pluripotent and Embryonic Stem Cells.- MicroRNA Expression During Neuronal Differentiation of Human Teratocarcinoma NTera2D1 and Mouse Embryonic Carcinoma P19 Cells.- Isolation and Identification of Gene-Specific MicroRNAs.- Transgene-Like Animal Models Using Intronic MicroRNAs.- Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR302 Induction.- Salivary MicroRNAs and Oral Cancer Detection.- Application of Intronic MicroRNA Agents in Cosmetics.- MicroRNAs in Skin and Wound Healing.- MiRNA Targets of Prostate Cancer.

• Format: Hardcover
• Language: English
• ISBN: 1627030824
• ISBN13: 9781627030823
• Release Date: September 2012
• Publisher: Humana
• Length: 375 Pages
• Weight: 1.90 lbs.
• Dimensions: 1.1" x 7.0" x 10.1"