Gene Expression Profiling: Methods and Protocols

Book Overview

1. Real-Time PCR and Multiplex Approaches

Olga L. Gurvich and Mikhail Skoblov

2. Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs

Sweta Rani and Lorraine O'Driscoll

3. Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals

Jai Prakash Mehta

4. Software and Tools for Microarray Data Analysis

Jai Prakash Mehta and Sweta Rani

5. Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumor Cells

Anne M. Friel, John Crown, and Lorraine O'Driscoll

6. Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using the Whole-Genome DASL Assay

Craig S. April and Jian-Bing Fan

7. MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs

Erica Hennessy and Lorraine O'Driscoll

8. Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases

Serena Germano and Lorraine O'Driscoll

9. 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins

Paula Meleady

10. Design, Construction, and Analysis of Cell Line Arrays and Tissue Microarrays for Gene Expression Analysis

Kathy Gately, Keith Kerr, and Ken O'Byrne

11. Immunohistochemical and Immunofluorescence Procedures for Protein Analysis

Kishore Reddy Katikireddy and Finbarr O'Sullivan

12. Advanced Microscopy: Laser Scanning Confocal Microscopy

Orla Hanrahan, James Harris, and Chris Egan

13. Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling

Sweta Rani, Keith O'Brien, Fergal C. Kelleher, Claire Corcoran, Serena Germano, Marek Radomski, John Crown, and Lorraine O'Driscoll

14. Atomic Force Microscopy (AFM) and High-Content Analysis (HCA): Two Innovative Technologies for Dissecting the Relationship Between Epithelial-Mesenchymal Transition (EMT)-Related Morphological and Structural Alterations and Cell Mechanical Properties

Stephen T. Buckley, Anthony M. Davies, and Carsten Ehrhardt

• Format: Paperback
• Language: English
• ISBN: 149396173X
• ISBN13: 9781493961733
• Release Date: August 2016
• Publisher: Humana
• Length: 211 Pages
• Weight: 0.87 lbs.
• Dimensions: 0.5" x 7.0" x 10.0"