Fundamentals of Light Microscopy and Electronic Imaging

By Douglas B. Murphy

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy

• Format: Hardcover
• Language: English
• ISBN: 047169214X
• ISBN13: 9780471692140
• Release Date: November 2012
• Publisher: Wiley-Blackwell
• Length: 560 Pages
• Weight: 3.25 lbs.
• Dimensions: 1.3" x 7.1" x 10.1"

Customer Reviews

A little dated, but a good textbook

I had to buy this book for a Graduate level course that I took. We covered all of the material in the book in about 4 weeks. I didn't know anything about the class beforehand, but the book did help a lot. It was a little dull, but the content was good albeit a little outdated.

Keep this book by the microscope

This is the book that tells you how to actually do effective microscopy. Keep a copy by the microscope.

Best Introductory-Level Textbook

Dr. Murphy constructs a solid foundation on the basic concepts of geometrical optics, light, and color, and then provides excellent introductory reviews of important topics in light microscopy. The book is very well written and complex phenomena are clearly explained without the unnecessary math that often confuses students. Illustrations are numerous and help support the text very nicely, as do the suggested laboratory exercises that accompany each chapter. Discussions of digital cameras and image processing are timely and provide the essential concepts necessary to tackle more advanced treatises. In the opinion of the Molecular Expressions microscopy website team, this book is by far the best entry-level textbook in the field.

Well Written and Useful

"This well written text provides a clear, uncluttered overview of the principles and practice of modern light microscopy. It contains many helpful teaching exercises and diagrams. It should prove useful in a wide range of courses from the undergraduate to the postgraduate level."-- Kenneth R. Spring, Ph.D., Author