Chikungunya Virus: Methods and Protocols

Book Overview

Part I Clinical and Diagnostic Virology

1. Evolution and Epidemiology of Chikungunya Virus
Giada Rossini, Maria Paola Landini, and Vittorio Sambri

2. Detection and Quantification of Chikungunya Virus by Real-Time RT-PCR Assay
Seok Mui Wang, Ummul Haninah Ali, Shamala Devi Sekaran, and Ravindran Thayan

3. Advanced Genetic Methodologies in Tracking Evolution and Spread of Chikungunya Virus
Hapuarachchige Chanditha Hapuarachchi and Kim-Sung Lee

4. Synthetic Peptide-Based Antibody Detection for Diagnosis of Chikungunya Infection with and without Neurological Complications
Rajpal S. Kashyap, Shradha S. Bhullar, Nitin H. Chandak, and Girdhar M. Taori

5. Expression and Purification of E2 Glycoprotein from Insect Cells (Sf9) for Use in Serology
Chong Long Chua, I-Ching Sam, and Yoke Fun Chan

6. Diagnostic Methods for CHIKV Based on Serological Tools
Paolo Gaibani, Maria Poala Landini, and Vittorio Sambri

7. Utilization and Assessment of Throat Swab and Urine Specimens for Diagnosis of Chikungunya Virus Infection
Chandrashekhar G. Raut, H. Hanumaiah, and Wrunda C. Raut

Part II Cell Culture, Virus Replication, and Cellular Responses

8. Propagation of Chikungunya Virus Using Mosquito Cells
Swee Kim Ang, Shirley Lam, and Justin Jang Hann Chu

9. Infectious Viral Quantification of Chikungunya Virus - Virus Plaque Assay
Parveen Kaur, Regina Ching Hua Lee, and Justin Jang Hann Chu

10. Molecular Epidemiology of Chikungunya Virus by Sequencing
Ravindran Thayan, Mohd Apandi Yusof, Zainah Saat, Shamala Devi Sekaran, and Seok Mui Wang

11. Chikungunya Virus Infection of Aedes Mosquitoes
Hui Vern Wong, Yoke Fun Chan, I-Ching Sam, Wan Yusof Wan Sulaiman, and Indra Vythilingam

12. Analysis of CHIKV in Mosquitoes Infected via Artificial Blood Meal
Jeremy P. Ledermann and Ann M. Powers

13. Chikungunya Virus Growth and Fluorescent Labeling: Detection of Chikungunya Virus by Immunofluorescence Assay
Meng Ling Moi and Tomohiko Takasaki

14. Virus Isolation and Preparation of Sucrose-Banded Chikungunya Virus Samples for Transmission Electron Microscopy
Chang-Kweng Lim

15. Viral-Host Protein Interaction Studies Using Yeast Two-Hybrid Screening Method
Namrata Dudha and Sanjay Gupta

16. Application of GelC-MS/MS to Proteomic Profiling of Chikungunya Virus Infection: Preparation of Peptides for Analysis
Atchara Paemanee, Nitwara Wikan, Sittiruk Roytrakul, and Duncan R. Smith

17. Bioinformatics Based Approaches to Study Virus-Host Interactions During Chikungunya Virus Infection
Sreejith Rajasekharan and Sanjay Gupta

18. T-Cell Epitope Prediction of Chikungunya Virus
Christine Loan Ping Eng, Tin Wee Tan, and Joo Chuan Tong

Part III Immunology and Animal Model Studies

19. Mouse Models of Chikungunya Virus
Lara J. Herrero, Penny A. Rudd, Xiang Liu, Stefan Wolf, and Suresh Mahalingam

20. Generation of Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HY Hybridoma Cloning Kit
Chow Wenn Yew and Yee Joo Tan

21. Immunohistochemical Detection of Chikungunya Virus Antigens in Formalin-Fixed and Paraffin-Embedded Tissues
Chun Wei Chiam, I-Ching Sam, Yoke Fun Chan, Kum Thong Wong, and Kien Chai Ong

• Format: Paperback
• Language: English
• ISBN: 1493980963
• ISBN13: 9781493980963
• Release Date: May 2018
• Publisher: Humana
• Length: 335 Pages
• Weight: 1.35 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"