Argonaute Proteins: Methods and Protocols

Book Overview

1. Purification of Native Argonaute Complexes from the Fission Yeast Schizosaccharomyces pombe

Shane M. Buker and Mohammad R. Motamedi

2. Chromatin Immuno-Precipitation in Fission Yeast

Thomas A. Volpe and Jessica DeMaio

3. Biochemical Analyses of Endogenous Argonaute Complexes Immunopurified with Anti-Argonaute Monoclonal Antibodies

Keita Miyoshi, Tomoko N. Okada, Haruhiko Siomi, and Mikiko C. Siomi

4. Mapping of Ago2-GW182 Functional Interactions

Bing Yao, Songqing Li, Shangli Lian, Marvin J. Fritzler, and Edward K.L. Chan

5. Continuous Density Gradients to Study Argonaute and GW182 Complexes Associated with the Endocytic Pathway

Derrick Gibbings

6. In vitro RISC Cleavage Assay

Julia Stoehr and Gunter Meister

7. Native Gel Analysis for RISC Assembly

Tomoko Kawamata and Yukihide Tomari

8. Purification and Assembly of Human Argonaute, Dicer, and TRBP Complexes

Nabanita De and Ian J. MacRae

9. Detection of Human Dicer and Argonaute 2 Catalytic Activity

Marjorie P. Perron, Patricia Landry, Isabelle Plante, and Patrick Provost

10. Imaging the Cellular Dynamics of Drosophila Argonaute Proteins

Jing Li, Nima Najand, Wendy Long, and Andrew Simmonds

11. ----Live Cell Imaging of Argonaute Proteins in Mammalian Cells

Justin M. Pare, Joaquin Lopez-Orozco, and Tom C. Hobman

12. Reporter-Based Assays for Analyzing RNA Interference in Mammalian Cells

Lydia V. McClure, Gil Ju Seo, and Christopher S. Sullivan

13. Artificial Tethering of Argonaute Proteins for Studying their Role in Translational Repression of Target mRNAs

Stephanie Eckhardt, Emilia Szostak, Zhaolin Yang, and Ramesh Pillai

14. An Efficient System For Let-7 MicroRNA- And GW182 Protein-Mediated Deadenylation In vitro

Marc R. Fabian, Yuri V. Svitkin, and Nahum Sonenberg

15. Cell-Free microRNA-Mediated Translation Repression in C. elegans

Edlyn Wu and Thomas F. Duchaine

16. Argonaute Pull-Down and RISC Analysis Using 2'-O-Methylated Oligonucleotides Affinity Matrices

Guillaume Jannot, Alejandro Vasquez-Rifo, and Martin J. Simard

17. Cloning Argonaute-Associated Small RNAs from C. elegans

Weifeng Gu, Julie M. Claycomb, Pedro J Batista, Craig C. Mello, and Darryl Conte, Jr.

18. Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs

Yohei Kirino, Anastassios Vourekas, Eugene Khandros, and Zissimos Mourelatos

19. Generation of an Inducible Mouse ES Cell Line Deficient for Argonaute Proteins

Hong Su and Xiaozhong Wang

20. Whole Cell Proteome Regulation by microRNAs Captured in a Pulsed SILAC Mass Spectrometry Approach

Olivia Ebner and Matthias Selbach

• Format: Paperback
• Language: English
• ISBN: 1493958275
• ISBN13: 9781493958276
• Release Date: August 2016
• Publisher: Humana
• Length: 344 Pages
• Weight: 1.37 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"