Trinucleotide Repeat Protocols

Book Overview

Part I. Introduction for Trinucleotide Repeat diseases

1. A Brief History of Triplet Repeat Disease

Helen Budworth and Cynthia McMurray

2. Huntington's Disease and Cell Therapies: Past, Present and Future

Chrystalina A. Antoniades and Colin Watts

Part II. Analysis of synaptic plasticity

3. Transcranial Two-Photon Imaging of Synaptic Structures in the Cortex of Awake Head-Restrained Mice

Guang Yang, Feng Pan, Paul C. Chang, Frank Gooden, and Wen-Biao Gan

4. In vivo Imaging of Synapse Plasticity in the Mouse Motor Cortex

Yi Zuo, Xinzhu Yu, Kelly Tennant, and Theresa Jones

Part III. Targeting on ES cells

5. Pluripotent Hybrid Stem Cells from Transgenic Huntington's Disease Monkey

Chuti Laowtammathron and Anthony W.S. Chan

6. Mesenchymal Stem Cells for Trinucleotide Repeat Disorders

Geralyn Annett, Gerhard Bauer, and Jan A. Nolta

Part IV. Targeting on RNA

7. Lentiviral-Mediated Gene Transfer of siRNAs for the Treatment of Huntington's Disease

Karine Cambon and Nicole D?glon

8. Purification of dFMR1-Containing Complexes Using Tandem Affinity Purification

Keita Miyoshi, Akiyo Ogino, Mikiko C. Siomi, and Haruhiko Siomi

9. Combined FISH and Immunofluorescent Staining Methods to Co-localize Proteins and mRNA in Neurons and Brain Tissue

Bin Ma and Naoko Tanese

Part V. Modification of Proteins and DNAs

10. Epigenetic Modifications of the FMR1 Gene

Elisabetta Tabolacci and Giovanni Neri

11. The CGG Repeat and the FMR1 Gene

Renate K. Hukema and Ben A. Oostra

12. Study of Oxidative Damage and Antioxidant Systems in Two Huntington s Disease Rodent Models

Francisca P?rez-Severiano, Sergio Montes-L?pez, Cristian Ger?nimo-Olvera, and Jos? Segovia

13. Regulation of Ataxin-1 Phosphorylation and Its Impact on Biology

Sarita Lagalwar and Harry T. Orr

Part VI. Trinucleotide repat expansion and detection

14. Towards Understanding Region-Specificity of Triplet Repeat Diseases: Coupled Immunohistology and Mass Spectrometry Imaging

Virginia Platt, Do Yup Lee, Christie Canaria, Ken Frankel, Susan Bernstein, and Cynthia McMurray

15. Antibodies and Intrabodies Against Huntingtin: Production and Screening of Monoclonals and Single-Chain Recombinant Forms

Ali Khoshnan, Susan Ou, Jan Ko, and Paul H. Patterson

16. Cell Recovery from DM1 Transgenic Mouse Tissue to Study (CTG)n Instability and DM1 Pathogenesis

Judith R. Brouwer, Laurent Foiry, and Genevi?ve Gourdon

17. Markerless Modification of Trinucleotide Repeat Loci in BACs

Kellie A. Benzow and Michael D. Koob

18. DRPLA: Recent Advances in Research Using Transgenic Mouse Models

Kazushi Suzuki, Toshiya Sato, Mitsunori Yamada, Hitoshi Takahashi, and Shoji Tsuji

• Format: Paperback
• Language: English
• ISBN: 1493959719
• ISBN13: 9781493959716
• Release Date: August 2016
• Publisher: Humana
• Length: 296 Pages
• Weight: 1.20 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"