Therapeutic Oligonucleotides: Methods and Protocols

Book Overview

1. Therapeutic Oligonucleotides

John Goodchild

2. Dinucleotides Containing 3[-S-Phosphorothiolate Linkages

James W. Gaynor and Richard Cosstick

3. 2'-O,4'-C-Methyleneoxymethylene Bridged Nucleic Acids (2',4'-BNACOC)

Yoshiyuki Hari, Tetsuya Kodama, Takeshi Imanishi, and Satoshi Obika

4. A Non-covalent Peptide-based Strategy for Ex-vivo and In vivo Oligonucleotide Delivery

Laurence Crombez, May C. Morris, Frederic Heitz, and Gilles Divita

5. Cell Penetrating Peptides-based Strategies for the Delivery of Splice Redirecting Antisense Oligonucleotides

Samir El Andaloussi, Fatouma Said Hassane, Prisca Boisguerin, Rannar Sillard, Ulo Langel, and Bernard Lebleu

6. A Nanoparticle for Tumor Targeted Delivery of Oligomers

Xinrong Liu, Yi Wang, and Donald J. Hnatowich

7. Light-directed Delivery of Nucleic Acids

Sigurd B e, Lina Prasmickaite, Birgit Enges ter, and Eivind Hovig

8. Antibody Targeted siRNA Delivery

Masoud M. Toloue and Lance P. Ford

9. Aptamer-Drug Conjugation for Targeted Tumor Cell Therapy

Michael J. Donovan, Ling Meng, Tao Chen, Yunfei Zhang, Kwame Sefah, and Weihong Tan

10. Five-step Process for Screening Antisense Compounds for Efficacy: Gene Target IL-12Rb2

Nikki B. Marshall, Laura L. Hauck, and Dan V. Mourich

11. Diverse Small Non-coding RNAs in RNA Interference Pathways

Liande Li and Yi Liu

12. Quantification of siRNAs In vitro and In vivo

Angie Cheng, Alexander V. Vlassov, and Susan Magdaleno

13. Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR

Angie Cheng, Susan Magdaleno, and Alexander V. Vlassov

14. siRNA Knockdown of Gene Expression in Endothelial Cells

Emily Dennstedt and Brad Bryan

15. Using RNA Interference in Schistosoma mansoni

Rita Bhardwaj, Greice Krautz-Peterson, and Patrick J. Skelly

16. Performing the Labeled microRNA Pull-down (LAMP) Assay System: An Experimental Approach for High-throughput Identification of microRNA-target mRNAs

Ren-Jun Hsu and Huai-Jen Tsai

17. Synthesis, Purification, and Characterization of Oligoribonucleotides that Act as Agonists of TLR7 and/or TLR8

Tao Lan and Ekambar R. Kandimalla

18. Synthesis, Purification, and Characterization of Immune-Modulatory Oligodeoxynucleotides that Act as Agonists of Toll-like Receptor 9

Mallikarjuna Reddy Putta, Dong Yu, and Ekambar R. Kandimalla

19. Surface Plasmon Resonance Investigation of RNA Aptamer-RNA Ligand Interactions

Carmelo Di Primo, Eric Dausse, and Jean-Jacques Toulm?

20. Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute Protein

Jacob C. Schwartz and David R. Corey

21. Inhibition of Human Papillomavirus Expression Using DNAzymes

Mar?a Luisa Ben?tez-Hess, Pablo Reyes-Guti?rrez, and Luis Marat Alvarez-Salas

• Format: Paperback
• Language: English
• ISBN: 1493958224
• ISBN13: 9781493958221
• Release Date: August 2016
• Publisher: Humana
• Length: 340 Pages
• Weight: 1.35 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"