The Low Molecular Weight Proteome: Methods and Protocols

Book Overview

Part I: Low Molecular Weight Analysis Methods

1. Narrow Range Peptide Iso Electric Focusing as Peptide Prefractionation Method Prior to Tandem Mass Spectrometry Analysis

Maria Pernemalm

2. Analysis of Peptides by Denaturing Ultrafiltration and LC-MALDI-TOF-MS

Yanming An and Radoslav Goldman

3. Stable Isotope Labeling Methods in Protein Profiling

Johan Lengqvist and AnnSofi Sandberg

4. Development of MRM-based Assays for the Absolute Quantitation of Plasma Proteins

Michael A. Kuzyk, Carol E. Parker, Dominik Domanski, and Christoph H. Borchers

5. Mass Spectrometric Profiling of Low-Molecular-Weight Proteins

Matthias Rainer, Constantin Sajdik, and G?nther K. Bonn

6. Developing an iMALDI Method

Brinda Shah, Jennifer D. Reid, Michael A. Kuzyk, Carol E. Parker, and Christoph H. Borchers

7. Analysis of Neuropeptides by MALDI Imaging Mass Spectrometry

Anna Karlsson, J?rg Hanrieder, Jonas Bergquist, and Malin Andersson

8. Highly Multiplexted Antibody Suspension Bead Arrays for Plasma Protein Profiling

By Kimi Drobin, Peter Nilsson and Jochen M. Schwenk

Part II: Bioinformatics of Proteomics Data

9. Protein Quantification by Peptide Quality Control (PQPQ) of Shotgun Proteomics Data

Jenny Forshed

10. Collection and Handling of Blood Specimens for Peptidomics

Harald Tammen and Rudiger Hess

Part III: Methods Applied in Clinical Research

11. An Automated RP-SCX Solid Phase Extraction Procedure for Urinary Peptidomics Biomarker Discovery Studies

Crina I.A. Balog, Rico Derks, Oleg A. Mayboroda and Andr? M. Deelder

12. Application of Phage Display for Ligand Peptidomics to Identify Peptide Ligands Binding to AQP2-expressing Membrane Fractions

Byung-Heon Lee and Tae-Hwan Kwon

13. Protein Expression Profiling of Brain Tumor Tissue Using SELDI-MS

Carl Wibom

14. Comprehensive Analysis of MHC Ligands in Clinical Material by Immunoaffinity - Mass Spectrometry

Kie Kasuga

• Format: Paperback
• Language: English
• ISBN: 1493955675
• ISBN13: 9781493955671
• Release Date: August 2016
• Publisher: Springer
• Length: 223 Pages
• Weight: 0.92 lbs.
• Dimensions: 0.5" x 7.0" x 10.0"