The Escrt Complexes: Methods and Protocols

Book Overview

1. Analysis of the Archaeal ESCRT Apparatus

Rachel Y. Samson, Iain G. Duggin, and Stephen D. Bell

2. Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster

Marco Gualtieri and Thomas Vaccari

3. Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc

Tamas Matusek, Pascal Th?rond, and Maximilian F?rthauer

4. Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative-Light Electron Microscopy

C?line Largeau, Emmanuel Culetto, and Renaud Legouis

5. Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells

Binod Kumar, Mohanan Valiya Veettil, Arunava Roy, and Bala Chandran

6. Immuno-Localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy

Keisuke Tabata, Atsuki Nara, Hiroko Omori, and Eiji Morita

7. Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking

Jalal M. Kazan, Gergely L. Lukacs, Pirjo M. Apaja, and Arnim Pause

8. Genetic and Biochemical Analyses of Yeast ESCRT

Sudeep Banjade, Shaogeng Tang, and Scott D. Emr

9. Live Imaging of ESCRT Proteins in Microfluidically-Isolated Hippocampal Axons

Veronica Birdsall, Jose C. Martinez, Lisa Randolph, Ulrich Hengst, and Clarissa L. Waites

10. Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High Resolution Imaging Techniques SIM and Cryo-SXT

Shai Adar-Levor, Inna Goliand, Michael Elbaum, and Natalie Elia

11. Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software

Romain Le Bars, Michele W. Bianchi, and Christophe Lefebvre

12. Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts

Marie-Kristin Nagel, Karin Vogel, and Erika Isono

13. Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins

Takayuki Obita, Rieko Kojima, and Mineyuki Mizuguchi

14. Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro

Samuel Block, Amber L. Schuh, and Anjon Audhya

15. Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays

Luisa Capalbo, Ioanna Mela, Maria Alba Abad, A. Arockia Jeyaprakash, J. Michael Edwardson, and Pier Paolo D'Avino

• Format: Paperback
• Language: English
• ISBN: 1493994948
• ISBN13: 9781493994946
• Release Date: August 2020
• Publisher: Humana
• Length: 322 Pages
• Weight: 1.30 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"