The Bacterial Flagellum: Methods and Protocols

Book Overview

Preface...
Table of Contents...
Contributors...

Part I Flagellar Type III Protein Export, Assembly and Gene Regulation in Salmonella enterica

1. Fuel of the Bacterial Flagellar Type III Protein Export Apparatus
Tohru Minamino, Miki Kinoshita and Keiichi Namba

2. Interactions of Flagellar Structural Subunits with the Membrane Export Components Machinery
Lewis D.B. Evans, Paul M. Bergen, Owain J. Bryant and Gillian M. Fraser

3. Fluorescent Microscopy Techniques to Study Hook Length Control and Flagella Formation
Marc Erhardt

4. Coupling of Flagellar Gene Expression with Assembly in Salmonella enterica
Fabienne F.V. Chevance and Kelly T Hughes

5. Dynamic Measures of Flagellar Gene Expression
Santosh Koirala and Christopher V. Rao

Part II Structure of the Bacterial Flagellum

6. Purification and Characterization of the Bacterial Flagellar Basal Body from Salmonella enterica
Shin-Ichi Aizawa

7. Design and Preparation of the Fragment Proteins of the Flagellar Components Suitable for X-Ray Crystal Structure Analysis
Katsumi Imada

8. Structural Analysis of the Flagellar Component Proteins in Solution by Small Angle X-Ray Scattering
Lawrence K. Lee

9. Structural Study of the Bacterial Flagellar Basal Body by Electron Cryomicroscopy and Image Analysis
Akihoro Kawamoto and Keiichi Namba

10. Structure of the MotA/B Proton Channel
Akio Kitao and Yasutaka Nishihara

11. Mechanism of Stator Assembly and Incorporation into the Flagellar Motor
Seiji Kojima

Part III Dynamics of the Bacterial Flagellar Motor

12. Rotation Measurements of Tethered Cells
Yuichi Inoue

13. Tracking the Movement of a Single Prokaryotic Cell in Extreme Environmental Conditions
Masayoshi Nishiyama and Yoshiyuki Arai

14. Measurements of the Rotation of the Flagellar Motor by Bead Assay
Taishi Kasai and Yoshiyuki Sowa

15. Measurements of Ion-Motive Force Across the Cell Membrane
Tsai-Shun Lin, Yi-Ren Sun, and Chien-Jung Lo

16. Stoichiometry and Turnover of the Stator and Rotor
Yusuke V. Morimoto and Tohru Minamino

17. Direct Imaging of Intracellular Signaling Molecule Responsible for the Bacterial Chemotaxis
Hajime Fukuoka

Part IV Structural Diversity of the Bacterial Flagellar Motors Derived from Different Bacterial Species

18. In Situ Structural Analysis of the Spirochetal Flagellar Motor by Cryo-Electron Tomography
Shiwei Zhu, Zhuan Qin, Juyu Wang, Dustin R. Morado, and Jun Liu

19. Motility of Spirochetes

20. Structure of the Sodium-Driven Flagellar Motor in Marine Vibrio
Yasuhiro Onoue and Michio Homma

21. Chemotactic Behaviors of Vibrio Cholerae Cells
Ikuro Kawagishi and So-ichiro Nishiyama

22. Purification of Fla2 Flagella of Rhodobacter sphaeroides
Javier de la Mora, Laura Camarena, and Georges Dreyfus

23. Dynamics in the Dual Fuel Flagellar Motor of Shewanella oneidensis MR-1
Susanne Brenzinger and Kai M. Thormann

24. Ion Selectivity of the Flagellar Motors Derived from the Alkaliphilic Bacillus and Paenibacillus Species
Yuka Takahashi and Masahiro Ito

25. Measurement of Free-Swimming Motility and Magnetotactic Behavior of Magnetococcus massalia Strain MO-1
Wei-Jia Zhang, Sheng-Da Zhang, and Long-Fei Wu

• Format: Hardcover
• Language: English
• ISBN: 1493969269
• ISBN13: 9781493969265
• Release Date: April 2017
• Publisher: Humana
• Length: 326 Pages
• Weight: 1.78 lbs.
• Dimensions: 0.8" x 7.0" x 10.0"