Sumo: Methods and Protocols

Book Overview

Part I Introduction

1. Concepts and Methodologies to Study Protein SUMOylation: An Overview
Michael J. Matunis and Manuel S. Rodr?guez

2. The Regulation of Chromatin by Dynamic SUMO Modifications
Nicole R. Wilson and Mark Hochstrasser

Part II Procedures to Study Protein SUMOylation In Vitro

3. Reconstitution of the Recombinant RanBP1 SUMO E3 Ligase Complex
Tobias Ritterhoff, Hrishikesh Das, Yuqing Hao, Volkan Sakin, Annette Flotho, Andreas Werner, and Frauke Melchior

4. Production and Purification of Recombinant SUMOylated Proteins Using Engineered Bacteria

Fr?d?rique Brockly, Marc Piechaczyk, and Guillaume Bossis
5. A Fluorescent In Vitro Assay to Investigate Paralog Specific SUMO ConjugationNathalie Eisenhardt, Viduth K. Chaugule, and Andrea Pichler

6. Identification and Characterization of SUMO-SIM Interactions
Koraljka Husnjak, Jan Keiten-Schmitz, and Stefan M?ller

7. Real-Time Surface Plasmon Resonance (SPR) for the Analysis of Interactions between SUMO-Traps and Mono or PolySUMO Moieties
Wendy Xolalpa, Manuel S. Rodr?guez, and Patrick England

8. Using Biotinylated SUMO-Traps to Analyze SUMOylated Proteins
Val?rie Lang, Elisa Da Silva-Ferrada, Rosa Barrio, James D. Sutherland, and Manuel S. Rodr?guez

9. In Vitro Characterization of Chain Depolymerization Activities of SUMO-Specific ProteasesJulia Eckhoff and R. J?rgen Dohmen
Part III Analysis of Protein SUMOylation Using Cell Lines

10. Detection of Protein SUMOylation In Situ by Proximity Ligation AssaysUmut Sahin, Florence Jollivet, Caroline Berthier, Hugues de Th?, and Val?rie Lallemand-Breitenbach

11. In Situ SUMOylation and DeSUMOylation Assays: Fluorescent Methods to Visualize SUMOylation and DeSUMOylation in Permeabilized CellsEri Yuasa and Hisato Saitoh
12. Analysis of SUMOylated Proteins in Cells and In Vivo Using BioSUMO StrategyLucia Pirone, Wendy Xolalpa, Ugo Mayor, Rosa Barrio, and James D. Sutherland
13. Label-Free Identification and Quantification of SUMO Target ProteinsIvo A. Hendriks and Alfred C.O. Vertegaal
14. The Use of Multimeric Protein Scaffolds for Identifying Multi-SUMO Binding ProteinsElisa Aguilar-Mart?nez and Andrew D. Sharrocks
15. Isolation of In Vivo SUMOylated Chromatin-Bound ProteinsTasneem Bawa-KhalfePart IV Procedures to Study Protein SUMOylation In Vivo
16. Identification of Substrates of Protein-Group SUMOylationIvan Psakhye and Stefan Jentsch
17. Tools to Study SUMO Conjugation in Caenorhabditis elegansFederico Pelisch and Ronald T. Hay
18. Purification of SUMO Conjugates from Arabidopsis for Mass Spectrometry AnalysisTh?r?se C. Rytz, Marcus J. Miller, and Richard D. Vierstra
19. Detection of SUMOylation in Plasmodium falciparumKatherine H. Reiter and Michael J. Matunis
20. Systematic Localization and Identification of SUMOylation Sub

• Format: Paperback
• Language: English
• ISBN: 1493981757
• ISBN13: 9781493981755
• Release Date: April 2018
• Publisher: Humana
• Length: 306 Pages
• Weight: 1.23 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"