Studies on the Confocal Laser Microscope

By Abdallah Mohamed Hamed

This book provides a thorough exploration of various modulated apertures and their impact on improving microscope resolution, with a focus on confocal scanning laser microscopy (CSLM). Over the course of eleven chapters, it looks at both the theoretical aspects and practical applications of different aperture shapes. Chapters 1 and 2 review apertures with linear, quadratic, and concentric black-and-white (B/W) zones, along with linear-quadratic and polynomial designs. Additionally, apertures with Hamming, Cauchy, rectangular, and hexagonal shapes are analyzed for their potential to enhance imaging performance. Chapter 3 presents the computation of coherent transfer functions (CTFs) for selected modulated apertures, offering insights into their influence on imaging quality. Chapter 4 focuses on confocal microscopes, exploring how these apertures affect the imaging of microscopic objects. A theoretical study of coherent non-scanned laser microscopes (CNSM) is covered in Chapter 5. Chapter 6 addresses the computation of lateral and axial point spread functions (PSFs) in confocal imaging systems that use binary amplitude masks, while Chapter 7 investigates the effects of misalignment errors combined with wavefront aberrations in systems using linear and quadratic apertures. In Chapter 8, diffraction intensity is calculated for a confocal microscope with a laterally displaced truncated Gaussian aperture, extending the principles of Marechal microscopy to confocal scanning microscopy. Chapters 9 and 10 examine spatial coherence in confocal optical systems, particularly in the context of quadratic and concentric B/W apertures. The book concludes in Chapter 11 with an application of cardiac apertures in CSLM, demonstrating their use in processing cardiac images. This work serves as a valuable reference for researchers and professionals interested in advancing microscope resolution through innovative aperture design and analysis.

• Format: Paperback
• Language: English
• ISBN: 3031872746
• ISBN13: 9783031872747
• Release Date: March 2025
• Publisher: Springer
• Length: 143 Pages
• Weight: 0.63 lbs.
• Dimensions: 0.3" x 6.2" x 9.3"