Root Development: Methods and Protocols

Book Overview

Part I: Genetic and Phenotypic Analyses

1. Measuring Plant Root Traits Under Controlled and Field Conditions: Step-By-Step Procedures

Benjamin M. Delory, Emanuela W.A. Weidlich, Richard van Duijnen, Lo?c Pag?s, and Vicky M. Temperton

2. Phenotyping Crop Root Crowns: General Guidance and Specific Protocols for Maize, Wheat, and Soybean

Larry M. York

3. Developmental Analysis of Arabidopsis Root Meristem

Riccardo Di Mambro and Sabrina Sabatini

4. Genetic and Phenotypic Analysis of Lateral Root Development in Arabidopsis thaliana

Selene Napsucialy-Mendivil and Joseph G. Dubrovsky

5. Adapting the Lateral Root Inducible System to Medicago truncatula

Violaine Herrbach, Fabienne Maillet, and Sandra Bensmihen

6. Characterization of Root Epidermal Cell Patterning and differentiation in Arabidopsis

Jorge E. Salazar-Henao, Girish Mokkapati, Eunice Han Xian Khor, Yi-Chia Chou, Wann-Neng Jane, and Wolfgang Schmidt

7. In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls

Hoang Khai Trinh, Inge Verstraeten, and Danny Geelen

8. Root Gravitropism: Quantification, Challenges, and Solutions

Lukas Muller, Malcolm J. Bennett, Andy French, Darren M. Wells, and Ranjan Swarup

Part II: Microscopy and Cell Biology

9. Calcium Ion Dynamics in Roots: Imaging and Analysis

Ellie Himschoot, Melanie Krebs, Alex Costa, Tom Beeckman, and Steffen Vanneste

10. Optimized Whole-Mount In Situ Immunolocalization for Arabidopsis thaliana Root Meristems and Lateral Root Primordia

Michael Karampelias, Ricardo Tejos, Jiř? Friml, and Steffen Vanneste

11. --Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development

Paolo Baesso, Ricardo S. Randall, and Giovanni Sena

12. Histological Profiling Over Time to Optimize Root Cell Type-Specific Reporter Lines for Cell Sorting

Beatriz Lagunas, Liam Walker, Rana Muhammad Fraz Hussain, Ian Hands-Portman, Kate Woolley-Allen, and Miriam L. Gifford

13. Long-Term In Vivo Imaging of Luciferase-Based Reporter Gene Expression in Arabidopsis Roots

Wei Xuan, Davy Opdenacker, Steffen Vanneste, and Tom Beeckman

14. Cortical Cell Length Analysis During Gravitropic Root Growth

Maria Sch?ller, J?rgen Kleine-Vehn, and Elena Feraru

15. Growth Rate Normalization Method to Assess Gravitropic Root Growth

Maria Sch

• Format: Hardcover
• Language: English
• ISBN: 1493977466
• ISBN13: 9781493977468
• Release Date: March 2018
• Publisher: Humana
• Length: 324 Pages
• Weight: 1.78 lbs.
• Dimensions: 0.8" x 7.0" x 10.0"