RNA: Methods and Protocols

Book Overview

Part I: RNA Methods 1. The Transcriptional Landscape Henrik Nielsen 2. Working with RNA Henrik Nielsen 3. Synthesis of RNA by In vitro Transcription Bertrand Beckert and Benoit Masquida 4. Efficient Poly(A)+ RNA Selection Using LNA Oligo(T) Capture Nana Jacobsen, Jens Eriksen, and Peter Stein Nielsen 5. Genome Browsers Elfar Torarinsson 6. Web-Based Tools for Studying RNA Structure and Function Ajish D. George and Scott A. Tenenbaum 7. Northern Blotting Analysis Knud Josefsen and Henrik Nielsen 8. Rapid Amplification of cDNA Ends (RACE) Oladapo Yeku and Michael A. Frohman 9. Fractionation of mRNA Based on the Length of the Poly(A) Tail Hedda A. Meijer and Cornelia H. de Moor 10. Analysis of Mutations that Influence Pre-mRNA Splicing Zhaiyi Zhang and Stefan Stamm 11. S1 Nuclease Analysis of Alternatively Spliced mRNA Martin L?tzelberger and J rgen Kjems 12. Promises and Challenges in Developing RNAi as a Research Tool and Therapy Mouldy Sioud 13. Inhibition of Gene Function in Mammalian Cells Using Short-Hairpin RNA (shRNA) J rn Remi Henriksen, Jochen Buechner, Cecilie L kke, Trond Fl gstad, and Christer Einvik 14. Validation of RNAi by Real Time PCR Knud Josefsen and Ying C. Lee 15. Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method Joel Nelson, Oleg Denisenko, and Karol Bomsztyk Part II: RNP Methods 16. The Post-Transcriptional Operon Scott A. Tenenbaum, Jan Christiansen, and Henrik Nielsen 17. RIP-Chip Analysis: RNA-Binding Protein Immunoprecipitation-Microarray (Chip) Profiling Ritu Jain, Tiffany Devine, Ajish D. George, Sridar V. Chittur, Timothy E. Baroni, Luiz O. Penalva, and Scott A. Tenenbaum 18. Isolation of RNP Granules Lars J nson, Finn Cilius Nielsen, and Jan Christiansen 19. Electrophoretic Mobility Shift Assay for Characterizing RNA-Protein Interaction Keith T. Gagnon and E. Stuart Maxwell 20. Polysome Analysis and RNA Purification from Sucrose Gradients Tom?s Masek, Leos Val?sek, and Martin Posp?sek 21. Prediction of Targets for microRNAs Morten Lindow 22. Outsourcing of Experimental Work Henrik Nielsen Also features electronic supplemental chapters: ESM1: In-Line Probing Analysis of Riboswitches Elizabeth E. Regulski and Ronald R. Breaker ESM2: Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2'-Hydroxyl Acylation and Primer Extension (SHAPE) Catherine A. Wakeman1 and Wade C. Winkler

• Format: Paperback
• Language: English
• ISBN: 1493956434
• ISBN13: 9781493956432
• Release Date: April 2017
• Publisher: Humana
• Length: 329 Pages
• Weight: 1.33 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"