This second edition provides new and up-to-date methods to study RNA chaperones in vitro and protocols for cell-based assays. Chapter guide readers through bacterial proteins, proteins from eukaryotic cells, complex interactions between RNA chaperones, and the folding and unfolding of proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, RNA Chaperones: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field
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