Recombinant Gene Expression

By Argelia Lorence

Table of Contents

"Recombinant Gene Expression: Reviews and Protocols, Third Edition"

Argelia Lorence (Editor)

Name of corresponding author(s) is underlined

General Aspects

1) Using Folding Promoting Agents in Recombinant Protein Production: A Review

Beatrix Fahnert

2) Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass Spectrometry

Brett J. Savary and Prasanna Vasu

3) A Matter of Packaging: Influence of Nucleosome Positioning on Heterologous Gene Expression

Mar?a de la Cruz Mu?oz-Centeno, Gonzalo Mill?n-Zambrano and Sebasti?n Chavez

4) Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant Proteins

Maureen C. Dolan, Giuliana Medrano, Anthony McMickle, and Carole L. Cramer

Prokaryotes

5) Heat-shock Protein Fusion Vectors for Improved Expression of Soluble Recombinant Proteins in Escherichia coli

Christos A. Kyratsous and Christos A. Panagiotidis

6) The use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in Salmonella

Ferenc Vonderviszt, Rachel Sajo, Jozsef Dobo, Peter Zavodoszky

7) Optimization of Purification Protocols Based on the Step-by-step Monitoring of the Protein Aggregates in the Soluble Fractions

Ario De Marco

8) Heterologous Protein Expression by Lactococcus lactis

Julio Villatoro-Hern?ndez, Oscar Paul Kuipers, Odila Saucedo-C?rdenas and Roberto Montes de Oca Luna

9) An Extended Suite of Genetic Tools for Use in Bacteria of the Halomonadaceae: An Overview

Monserrat Argando?a, Carmen Vargas, Javier Rodr?guez-Moya, Mercedes Reina-Bueno, Manuel Salvador and Joaquin J. Nieto

10) Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125

Valentina Rippa, Rosanna Papa, Maria Giuliani, Cinzia Pezzella, Ermenegilda Parrilli, Maria Luisa Tutino, Gennaro Marino, and Angela Duilio

11) A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125

Maria Giuliani, Ermenegilda Parrilli , Cinzia Pezzella, Valentina Rippa, Angela Duilio, Gennaro Marino and Maria Luisa Tutino

12) A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein-protein Interaction

Jumei Zeng and Zheng-Guo He

13) Heterologous High-level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatus

Nadine Katzke, Ren? Bergmann, Karl-Erich Jaeger Thomas Drepper

14) Plasmid DNA Production for Therapeutic Applications

Alvaro Lara and Octavio T. Ramirez

Lower eukaryotes

15) Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review

Tomoaki Niimi

16) Expression of Multi-subunit Proteins in Leishmania tarentolae

Marisa Sugino and Tomoaki Niimi

Fungi

17) Recombinant Protein Production in Yeasts

Diethard Mattanovich, Paola Branduardi, Laura Dato, Brigitte Gasser, Michael Sauer and Danilo Porro

18) Yeasts as Tool for Heterologous Gene Expression

Raja Mokdad-Gargouri, Salma Abdelmoula-Soussi, Nadia Hadiji-Abb?s, Ines Yacoubi-Hadj Amor, Istabrak Borchani-Chabchoub and Ali Gargouri

19) The Cre/lox system: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal Endophytes

Simona Florea, Caroline Machado, Kalina Andreeva, and Christopher L. Schardl

20) Aptamer-regulated Expression

• Format: Paperback
• Language: English
• ISBN: 1493962213
• ISBN13: 9781493962211
• Release Date: August 2016
• Publisher: Humana
• Length: 649 Pages
• Weight: 2.51 lbs.
• Dimensions: 1.3" x 7.0" x 10.0"