Rapid Cycle Real-Time PCR -- Methods and Applications: Genetics and Oncology

Book Overview

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting...

• Format: Paperback
• Language: English
• ISBN: 3642639658
• ISBN13: 9783642639654
• Release Date: September 2011
• Publisher: Springer
• Length: 205 Pages