Proteomics in Practice: A Guide to Successful Experimental Design

By Tom Naven, Reiner Westermeier, Hans-Rudolf Hopker

Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.

• Format: Hardcover
• Language: English
• ISBN: 3527319417
• ISBN13: 9783527319411
• Release Date: May 2008
• Publisher: Wiley-Blackwell
• Length: 502 Pages
• Weight: 1.05 lbs.
• Dimensions: 1.2" x 6.9" x 9.6"