Protein Phosphorylation : A Practical Approach

By Grahame Hardie

Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphorylation.

• Format: Paperback
• Language: English
• ISBN: B007YXP970
• ISBN13: 9780199637287
• Release Date: January 2000
• Publisher: Oxford University Press, Incorporated
• Length: 460 Pages
• Weight: 1.76 lbs.
• Dimensions: 1.0" x 6.1" x 9.2"