Protein Phosphorylation: A Practical Approach

By Grahame Hardie

Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphorylation.

• Format: Paperback
• Language: English
• ISBN: 0199637288
• ISBN13: 9780199637287
• Release Date: December 1999
• Publisher: Oxford University Press, USA
• Length: 464 Pages
• Weight: 1.58 lbs.
• Dimensions: 1.1" x 6.4" x 9.2"