Protein NMR Techniques

By Alexander Shekhtman

Book Overview

Table of Contents

1. A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-labeled Proteins

2 Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis

3. Isotope Labeling in Insect Cells

4. Isotope Labeling in Mammalian Cells

5. Cell-free Protein Production for NMR Studies

6. Cell-free Membrane Protein Expression for Solid-state NMR

7. Expression and Purification of Src-family Kinases for Solution NMR Studies

8. NMR Studies of Large Protein Systems

9. Protein Dynamics by 15N Nuclear Magnetic Relaxation

10. Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel

11. Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy

12. NMR Studies of Protein-RNA Interactions

13. Preparation and Optimization of Protein-DNA Complexes Suitable for Detailed NMR Studies

14. NMR Studies of Protein-ligand Interactions

15. In-cell NMR Spectroscopy in Escherichia coli

16. Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS solid-state NMR

17. Solid-State NMR Spectroscopy of Protein Complexes

18. Synthesis, Purification and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy

19. Assignment of Backbone Resonances in a Eukaryotic Protein Kinase - ERK2

20. Electrostatics of Hydrogen Exchange for Analyzing Protein Flexibility

21. Fast Protein Backbone NMR Resonance Assignment using the BATCH Strategy

22. Comprehensive Automation for NMR Structure Determination of Proteins

23. ARIA for Solution and Solid-state NMR Protein NMR Techniques

24. Determining Protein Dynamics from 15N Relaxation Data by using DYNAMICS

• Format: Hardcover
• Language: English
• ISBN: 1617794791
• ISBN13: 9781617794797
• Release Date: December 2011
• Publisher: Humana
• Length: 518 Pages
• Weight: 2.55 lbs.
• Dimensions: 1.3" x 7.0" x 10.1"