
High Performance Liquid Chromatography (HPLC) is a common method for analyzing substances and is used by researchers to measure the amount of caffeine in coffee, for example, or the level of penicillin in a blood sample. The process involves separating a substance into its individual...

This book shows the chemist who is already familiar with the basics of HPLC how to develop HPLC separation methods for a wide variety of situations. It focuses mainly on reversed-phase separations of small-molecules, but also addresses development of normal-phase and ion-pair...