Plant Transposable Elements: Methods and Protocols

Book Overview

1. Historical Overview of Transposable Element Research

Peter A Peterson

2. Distinguishing Variable Phenotypes from Variegation Caused by Transposon Activities

Virginia Walbot

3. Using Transposons for Genetic Mosaic Analysis of Plant Development

Philip W. Becraft

4. Survey of Natural and Transgenic Gene Markers Used to Monitor Transposon Activity

Lakshminarasimhan Krishnaswamy, Thomas Peterson

5. Molecular Biology of Maize Ac/Ds Elements - an Overview

Katina Lazarow, My-Linh Du and Reinhard Kunze

6. Gene Tagging with Engineered Ds Elements in Maize

Yubin Li, Gregorio Segal, Qinghua Wang, and Hugo K. Dooner

7. Plant Regeneration Methods for Rapid Generation of a Large Scale of Ds Transposant Population in Rice

Yuan Hu Xuan, Jin Huang, Gihwan Yi, Dong-Soo Park, Soo Kwon Park, Moo Young Eun, Doh Won Yun, Gang-Seob Lee, Tae Ho Kim, Chang-deok Han

8. Isolation of Sequences Flanking Ac Insertion Sites by Ac Casting

Dafang Wang and Thomas Peterson

9. Regulation of the Mutator System of Transposons in Maize

Damon Lisch

10. Using MuDR/Mu Transposons in Directed Tagging Strategies

Virginia Walbot and Julia Q?esta

11. Genetic and Molecular Analysis of UniformMu Transposon Insertion Lines

Donald R. McCarty, Masaharu Suzuki, Charles Hunter, Wayne T. Avigne, and Karen E. Koch

12. Digestion-Ligation-Amplification (DLA): A Simple Genome Walking Method to Amplify Unknown Sequences Flanking Mutator (Mu) Transposons and Thereby Facilitate Gene Cloning

Sanzhen Liu, An-Ping Hsia, Patrick S. Schnable

13. Molecular Genetics and Epigenetics of CACTA Elements

Nina V. Fedoroff

14. Activation Tagging Using the Maize En-I Transposon System for the Identification of Abiotic Stress Resistance Genes in Arabidopsis

Amal Harb and Andy Pereira

15. Reverse Genetics in Rice using Tos17

Delphine Mieulet, Anne Di?vart, Ga?tan Droc, Nad?ge Lanau and Emmanuel Guiderdoni

16. Identification and Applications of the Petunia Class II Act1/dTph1 Transposable Element System

Tom Gerats, Jan Zethof, and Michiel Vandenbussche

17. Transposon Display: A Versatile Method for Transposon Tagging

Michiel Vandenbussche, Jan Zethof, and Tom Gerats

18. Massive Indexed Parallel Identification of Transposon Flanking Sequences

Michiel Vandenbussche, Jan Zethof, and Tom Gerats

19. Use of Next Generation Sequencing (NGS) Technologies for the Genome-wide Detection of Transposition

Moaine Elbaidouri, Cristian Chaparro and Olivier Panaud

20. Overview of Repeat Annotation and de novo Repeat Identification

Ning Jiang

21. Computational Methods for Identification of DNA Transposons

Ning Jiang

22. TEnest 2.0: Computational Annotation and Visualization of Nested Transposable Elements

Brent A. Kronmiller and Roger P. Wise

• Format: Paperback
• Language: English
• ISBN: 149396271X
• ISBN13: 9781493962716
• Release Date: August 2016
• Publisher: Humana
• Length: 324 Pages
• Weight: 1.31 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"