Plant Proteases and Plant Cell Death: Methods and Protocols

1 Expression and purification of the type I metacaspase from a cryptophyte Guillardia theta, GtMCA-I

Vida Strancar, Katarina Petra van Midden, Marina Klemenčič, Christiane Funk

2 Expression and purification of the type II metacaspase from a unicellular green alga Chlamydomonas reinhardtii

Igor Sabljic, Yong Zou, Marina Klemenčič, Christiane Funk, Jerry St?hlberg, Peter Bozhkov

3 Expression in Escherichia coli, refolding, and purification of plant aspartic proteases
Pedro Castanheira, Carla Almeida, Daniela Dias-Pedroso, Isaura Sim?es

4 Production of functional plant legumain proteases using the Leishmania tarentolae expression system

Elfriede Dall, Andreas Licht, Hans Brandstetter

5 Purification of His-tagged proteases from the apoplast of agroinfiltrated N. benthamiana

Mariana Schuster, Judith K. Paulus, Jiorgos Kourelis, Renier A. L. van der Hoorn

6 Identification of cognate protease/substrate pairs by use of class-specific inhibitors
Annick Stintzi, Nils St?hrwohldt, Stefanie Royek, Andreas Schaller

7 Peptide backbone modifications for the assessment of cleavage site relevance in precursors of signaling peptides
Xu Wang, Jens Pfannstiel, Annick Stintzi and Andreas Schaller

8 Detection of apoplastic protease inhibitors using convolution activity based proteome profiling

Andrea Passarge, Gunther Doehlemann, Johana C. Misas Villamil

9 Cysteine reactivity profiling to unveil redox regulation in phytopathogens

Kyoko Morimoto, Monika Stegmann, Farnusch Kaschani, Shabaz Mohammed, Renier A. L. van der Hoorn

10 Determination of caspase-like activities in roots by the use of fluorogenic substrates

Katarina Soln, Marina Klemenčič

11 Detection of damage-activated metacaspase activity by Western blot in plants

Simon Stael, Luke P. Miller, ?lvaro D. Fern?ndez-Fern?ndez, Frank Van Breusegem

12 Sensitive plant N-terminome profiling with HUNTER
Fatih Demir, Andreas Perrar, Melissa Mantz, Pitter F. Huesgen

13 Profiling sequence specificity of proteolytic activities using proteome-derived peptide libraries

Fatih Demir, Maithreyan Kuppusamy, Andreas Perrar, Pitter F. Huesgen

14 Monitoring Cell Death via Ion leakage and PAM Fluorometry
Nick Dunken, Lisa Mahdi, Rainer E. H?usler, Alga Zuccaro

15 Measuring and perturbing ferroptosis in plants

Ayelen M. Dist?fano, Fernanda Marchetti, Eduardo

• Format: Hardcover
• Language: English
• ISBN: 1071620789
• ISBN13: 9781071620786
• Release Date: May 2022
• Publisher: Humana
• Length: 299 Pages
• Weight: 1.67 lbs.
• Dimensions: 0.8" x 7.0" x 10.0"