Plant Mitochondria: Methods and Protocols

Book Overview

1. Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Huy Cuong Tran and Olivier Van Aken

2. Cell Type-Specific Isolation of Mitochondria in Arabidopsis

Cl?ment Boussardon and Olivier Keech

3. Isolation of Plant Mitochondria Using Affinity Purification

Franziska Kuhnert and Andreas P.M. Weber

4. Isolation of Highly Purified, Intact, and Functional Mitochondria from Potato Tubers Using a Two-in-One Percoll Density Gradient

Ian Max M ller and Allan G. Rasmusson

5. Integrity Assessment of Isolated Plant Mitochondria

Allan G. Rasmusson, Mengshu Hao, and Ian Max M ller

6. High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores

Brendan M. O'Leary, Glenda Guek Khim Oh, and A. Harvey Millar

7. Assessment of Respiratory Enzymes in Intact Cells by Permeabilization with Alamethicin

Allan G. Rasmusson, Ian Max M ller, and Susanne Widell

8. Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring-Mass Spectrometry (SRM-MS)

Xuyen Le, A. Harvey Millar, and Chun Pong Lee

9. Complexome Profiling of Plant Mitochondrial Fractions

Lucie Schr?der, Holger Eubel, and Hans-Peter Braun

10. High Throughput BN-PAGE for Mitochondrial Respiratory Complexes

Lilian Vincis Pereira Sanglard and Catherine Colas des Francs-Small

11. Assessment of Mitochondrial Protein Composition and Purity by Mass Spectroscopy

Anqiang Tang, Yimin Wang, and Nicolas L. Taylor

12. Mitochondrial GFP-Tagged Protein Localization Using Transient Transformations in Arabidopsis thaliana

Cristina Ruberti

13. Assessment of Mitochondrial Protein Topology and Membrane Insertion

Kerstin Sch?fer, Carina Engstler, Korbinian Dischinger, and Chris Carrie

14. Assessment of Protein Synthesis in Mitochondria Isolated from Rosette Leaves and Liquid Culture Seedlings of Arabidopsis

Malgorzata Kwasniak-Owczarek, Artur Tomal, and Hanna Janska

15. Markers for Mitochondrial ROS Status

Monika Ostaszewska-Bugajska, Anna Podg?rska, and Bożena Szal

16. Mass Spectrometry-Based Quantitative Cysteine Redox Proteome Profiling of Isolated Mitochondria Using Differential iodoTMT Labeling

Jonas Giese, J?rgen Eirich, Frederik Post, Markus Schwarzl?nder, and Iris Finkemeier

17. Analysis of the Plant Mitochondrial Transcripto

• Format: Paperback
• Language: English
• ISBN: 1071616552
• ISBN13: 9781071616550
• Release Date: September 2021
• Publisher: Humana
• Length: 342 Pages
• Weight: 1.37 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"