Plant Germline Development: Methods and Protocols

Book Overview

Part I: Plant Germline Development: From Investigation to Application

1. An Introduction to Male Germline Development

Hannes Vogler, Ueli Grossniklaus, and Afif Hedhly

2. Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants

Joann A. Conner and Peggy Ozias-Akins

Part II: Clearing, Imaging Techniques, and Immunolocalization to Study Cell Lineage, Cell Type Specificity, Cytoskeleton, Cell Cycle, Signaling, and Hormones

3. 3D Imaging of Whole-Mount Ovules at Cellular Resolution to Study Female Germline Development in Rice

Ethel Mendocilla Sato, Wenjing She, and C?lia Baroux

4. Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana

Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, and Tomokazu Kawashima

5. Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers

Mark A. Chamberlin and Shai J. Lawit

6. Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy

David Twell and Lynette Brownfield

7. In Vivo Ploidy Determination of Arabidopsis thaliana Male and Female Gametophytes

Burcu Nur Ke?eli, Nico De Storme, and Danny Geelen

8. Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins

Daniel Slane, Patrick B?rgel, and Martin Bayer

9. Live Cell Imaging of Auxin and Cytokinin Signaling in Maize Female Gametophytes

Antony M. Chettoor and Matthew M.S. Evans

10. Imaging Ca2+ Dynamics in Wild-Type and NADPH Oxidase-Deficient Mutant Pollen Tubes with Yellow Cameleon and Confocal Laser Scanning Microscopy

Christina Maria Franck, Jens Westermann, and Aur?lien Boisson-Dernier

11. Immunolocalization of AGPs and Pectins in Quercus suber Gametophytic Structures

M?rio Lu?s da Costa, Ana L?cia Lopes, Maria Isabel Amorim, and S?lvia Coimbra

Part III: Chromosome Spreading, Whole Mount In Situ, and Transmission Electron Microscopy (TEM)

12. Optimization of Cell Spreading and Image Quality for the Study of Chromosomes in Plant Tissues

Laksana Kantama, Erik Wijnker, and Hans de Jong

13. Whole Mount RNA-FISH on Ovules and Developing Seeds

Andrea Bleckmann and Thomas Dresselhaus

14. Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy

Peng-Fei Jia, Hong-Ju Li, and Wei-Cai Yang

15. Transmission Electron

• Format: Hardcover
• Language: English
• ISBN: 1493972855
• ISBN13: 9781493972852
• Release Date: September 2017
• Publisher: Humana
• Length: 422 Pages
• Weight: 2.14 lbs.
• Dimensions: 0.9" x 7.0" x 10.0"