Plant Fungal Pathogens: Methods and Protocols

Book Overview

1. Fungal ABC Transporter Deletion and Localization AnalysisAndriy Kovalchuk, Stefan Weber, Jeroen G. Nijland, Roel A.L. Bovenberg, and Arnold J.M. Driessen2. Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens Mediated Transformation

Rasmus John Normand Frandsen, Mette Frandsen, Henriette Giese

3. Activity-based Protein Profiling of Infected Plants

Farnusch Kaschani, Christian Gu, Renier A.L. van der Hoorn

4. The use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves

Lisong Ma, Ewa Lukasik, Fleur Gawehns and Frank L.W. Takken

5. Proteomic Techniques for Plant-Funal Interactions

Delphine Vincent, Kar-Chun Tan, Liam Cassidy, Peter Solomon and Richard Oliver

6. Identification of HR-inducing cDNAs from Plant Pathogens via a Gateway(R)-Compatible Binary Potato Virus X-Expression Vector

H. Peter van Esse

7. Freeze-Fracture of Infected Plant Leaves in Ethanol for Scanning Electron Microscopic Study of Fungal Pathogens

Jayma A. Moore and Scott A. Payne

8. Real-time PCR for the Quantification of Fungi in planta

Steven J. Klosterman

9. Using Non-homologous End-joining-deficient Strains for Functional Gene Analyses in Filamentous Fungi

Mark Arentshorst, Arthur F.J. Ram, Vera Meyer

10. Atomic Force Microscopy (AFM): A Tool for Studying Biophysical Surface Properties Underpinning Fungal Interactions with Plants and Substrates

Elizabeth Adams, Danielle Emerson, Sean Croker, Hye-Seon Kim, Shannon Modla, Seogchan Kang, Kirk Czymmek

11. Use of the Yeast Two-Hybrid System to Identify Targets of Fungal Effectors

Shunwen Lu

12. Characterization of Plant - Fungal Interactions Involving Necrotrophic Effector-Producing Plant Pathogens

Timothy L. Friesen and Justin D. Faris

13.Heterologous Production of Fungal Effectors in Pichia pastoris

Anja Kombrink

14. The Application of Laser Microdissection to Profiling Fungal Pathogen Gene Expression in planta

Wei-Hua Tang, Yan Zhang, and Jon Duvick

15. Metabolomics Protocols for Filamentous Fungi

Joel P.A. Gummer, Christian Krill, Lauren Du Fall, Ormonde D.C. Waters, Robert D. Trengove, Richard P. Oliver and Peter S. Solomon

16. Targeted Gene Replacement in Fungi using a Split-Marker Approach

Rubella S. Goswami

17.Massively Parallel Sequencing Technology in Pathogenic Microbes

Sucheta Tripathy and Rays H.Y. Jiang

18.Confocal Microscopy in Plant Pathogen Interactions

Adrienne R. Hardham

19.The use of Open Source Bioinformatics Tools to Dissect Transcriptomic Data

Benjamin M. Nitsche, Arthur F.J. Ram, Vera Meyer

20.Population Biology of Fungal Plant Pathogens

Zahi K. Atallah and Krishna V. Subbarao

21.Polyethylene Glycol (PEG)-mediated Transformation in Filamentous Fungal Pathogens

Zhaohui Liu and Timothy L. Friesen

22.In vitro Induction of Infection-related Hyphal Structures in Plant Pathogenic Fungi

W.R. Rittenour, S.D. Harris

23.Fungicide Resistance Assays for Fungal Plant Pathogens

Gary A. Secor and Viviana V. Rivera

24.Identification of Lipid-binding Effectors

Shiv D. Kale and Brett M. Tyler

25. In silico Identification and Characterization of Effector Catalogs

Ronnie de Jonge

26. Horizontal Transfer of Supernumerary Chromosomes in Fungi

H. Charlotte van der Does and Martijn Rep

27. The Induction of Mycotoxins by Trichothecene Producing Fusarium Species

Rohan Lowe, M?lanie Jubault, Gail Canning, Martin Urban and Kim E. Hammond-Kosack

28. Identifying Genes in Fusarium verticillioides Through Forward and Reverse Genetics

• Format: Hardcover
• Language: English
• ISBN: 1617795003
• ISBN13: 9781617795008
• Release Date: December 2011
• Publisher: Humana
• Length: 648 Pages
• Weight: 2.95 lbs.
• Dimensions: 1.7" x 7.0" x 10.2"