Pdz Mediated Interactions: Methods and Protocols

Book Overview

Preface...
Table of Contents...
Contributing Authors...

1. Identification of PDZ Interactions by Yeast Two-Hybrid
Monica Castro-Cruz, Marta Monserrat-Gomez, Jean-Paul Borg, Pascale Zimmermann, and Eric Bailly

2. Identification of PDZ Interactions by Affinity Purification and Mass Spectrometry Analysis
Avais M. Daulat, St?phane Audebert, M?nica Wagner, Luc Camoin, and Jean-Paul Borg

3. Identification of PDZ Interactions by Proteomic Peptide Phage Display
Susanne L?chow, Gustav Sundell, and Ylva Ivarsson

4. A Computational Protocol to Analyze PDZ/PBM Affinity Data Obtained by High-Throughput Holdup Assay
Pau Jan?, Lionel Chiron, Goran Bich, Gilles Trav?, and Yves Nomin?

5. Study of PDZ-Peptide and PDZ-Lipid Interactions by Surface Plasmon Resonance/BIAcore
Pascale Zimmermann and Antonio Luis Egea-Jimenez

6. PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations
C?lia Caillet-Saguy, S?bastien Br?l?, Nicolas Wolff, and Bertrand Raynal

7. Crystallographic Studies of PDZ Domain-Peptide Interactions of the Scribble Polarity Module
Janesha C. Maddumage, Bryce Z. Stewart, Patrick O. Humbert, and Marc Kvansakul

8. A Fluorescence-Based Assay to Determine PDZ-Ligand Binding Thermodynamics
Young Joo Sun and Ernesto J. Fuentes

9. Unveiling the Folding Mechanism of PDZ Domains
Candice Gautier and Stefano Gianni

10. Development of Peptide-Based PDZ Domain Inhibitors
Dominik J. Essig, Javier R. Balboa, and Kristian Str mgaard

11. Dynamic Control of Signaling by Phosphorylation of PDZ Binding Motifs
M?rton A. Simon and L?szl? Nyitray

12. Chemical Synthesis of PDZ Domains
Christin Kossmann, Sana Ma, Louise S. Clemmensen, and Kristian Str mgaard

13. Viral PDZ Binding Motifs Influence Cell Behavior through the Interaction with Cellular Proteins Containing PDZ Domains
Carlos Casta?o-Rodriguez, Jose M. Honrubia, Javier Guti?rrez-?lvarez, Isabel Sola, and Luis Enjuanes

14. Computational Design of PDZ: peptide Binding
Nicolas Panel, Francesco Villa, Vaitea Opuu, David Mignon, and Thomas Simonson

15. Mechanoregulation of PDZ Proteins, An Emerging Function
Elsa Bazelli?res and Andr? Le Bivic

16. Rational Design of PDZ Domain Inhibitors: Discovery of Small Organic Compounds Targeting PDZ Domains
Laurent Hoffer, Philippe Roche, and Xavier Morelli

Subject Index List...

• Format: Paperback
• Language: English
• ISBN: 1071611682
• ISBN13: 9781071611685
• Release Date: May 2021
• Publisher: Humana
• Length: 292 Pages
• Weight: 1.18 lbs.
• Dimensions: 0.6" x 7.0" x 10.0"