PCR: Methods and Protocols

Book Overview

1. In silico PCR Tools for a Fast Primer, Probe and Advanced Searching

Ruslan Kalendar, Alexandr Muterko, Malika Shamekova, and Kabyl Zhambakin

2. Introduction on using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis

Ruslan Kalendar and Timofey V. Tselykh

3. Long Fragment Polymerase Chain Reaction

Eng Wee Chua, Simran Maggo, and Martin A. Kennedy

4. Strategies to Improve Efficiency and Specificity of Degenerate Primers in PCR

Maria Jorge Campos and Alberto Quesada

5. Inverse PCR for Point Mutation Introduction

Diogo Silva, Gustavo Santos, M?rio Barroca, and Tony Collins

6. Synthesis of Fusion Genes for Cloning by Megaprimer-based PCR

Tatiana Q. Aguiar, Carla Oliveira, and Luc?lia Domingues

7. A Novel Platform for High-throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli

Ana Filipa Sequeira, Joana L.A. Br?s, V?nia O. Fernandes, Catarina I.P.D. Guerreiro, Renaud Vincentelli, and Carlos M.G.A. Fontes

Fl?vio Azevedo, Humberto Pereira, and Bj?rn Johansson

9. CRENAME, a Molecular Microbiology Method Enabling Multiparametric Assessment of Potable/Drinking Water

Luc Bissonnette, Andr?e F. Maheux, and Michel G. Bergeron

10. Multiplex Detection Of Food-borne Pathogen

Germ?n Villamizar-Rodr?guez and Felipe Lomb?

11. Fast Real-time PCR for the Detection of Crustacean Allergens in Foods

Francisco J. Santaclara and Montserrat Espi?eira

12. Fast Real-time PCR Method for the Detection of Soy in Foods

Montserrat Espi?eira and Francisco J. Santaclara

13. RAPD/SCAR Approaches for Identification of Adulterant's Breed's Milk in Dairy Products

Joana Cunha and Luc?lia Domingues

14. Genetic Diversity Analysis of Medicinally Important Horticultural Crop Aegle marmelos by ISSR Markers

Farina Mujeeb, Preeti Bajpai, Neelam Pathak, and Smita Rastogu Verma

15. PCR in the Analysis of Clinical Samples: Prenatal and Postnatal Diagnosis of Inborn Errors of Metabolism

Laura Vilarinho and C?lia Nogueira

16. Harnessing the Power of PCR-dependent Molecular Fingerprinting Methods for Understanding Structure and Function in Microbial Communities

S.S. Phadke, A.F. Salvador, J.I. Alves, O. Bretschger, M.M. Alves, and M.A. Pereira

17. PCR in Metagenomics

Sarita G Bhat and Tina K. J.

18. Arbitrarily Primed PCR for Comparison of Metagenomes and Extracting useful loci from them

Leigh Burgoyne, Lin Y Koh, and David Catcheside

• Format: Paperback
• Language: English
• ISBN: 1493983814
• ISBN13: 9781493983810
• Release Date: August 2018
• Publisher: Springer
• Length: 282 Pages
• Weight: 0.92 lbs.
• Dimensions: 0.6" x 6.1" x 9.2"