Osteoporosis and Osteoarthritis

Book Overview

Part I: Cellular and Molecular Biology of Osteoarthritis and Osteoporosis

1. Isolation of Murine and Human Osteocyte

Matthew Prideaux, Amber Rath Stern, and Lynda F. Bonewald

2. Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

Roberto Narcisi, Wendy J.L.M. Koevoet, and Gergo J.V.M. van Osch

3. A Novel Enzymatic Digestion Approach for Isolation and Culture of Rodent Bone Marrow Mesenchymal Progenitors

Leilei Zhong, Lutian Yao, and Ling Qin

4. Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc

Guus G.H. van den Akker, Andy Cremers, Donatus A.M. Surtel, Willem Voncken, and Tim J.M. Welting

5. Engineering Cartilage Tissue by Co-Culturing of Chondrocytes and Mesenchymal Stromal Cells

Yao Fu, Carlo A. Paggi, Amel Dudakovic, Andre J. van Wijnen, Janine Post, and Marcel Karperien

6. Generation of Induced Pluripotent Stem Cells

David R. Deyle

7. Specimen Preparation for Single Cell Sequencing Analysis of Skeletal Cells

Shawon Debnath and Matthew Greenblatt

8. Mapping 5-Hydroxymethylcytosine (5hmC) Modifications in Skeletal Tissues Using High-Throughput Sequencing

Fiorella Carla Grandi and Nidhi Bhutani

9. Using FRAP to Quantify Changes in Transcription Factor Dynamics after Cell Stimulation: Cell Culture, FRAP, Data Analysis, and Visualization

Kannan Govindaraj and Janine N. Post

10. Quantitative Molecular Models for Biological Processes: Modeling of Signal Transduction Networks with ANIMO

Sakshi Khurana, Janet Huisman, Stefano Schivo, and Janine N. Post

Part II: In Vivo Models of Skeletal Tissue Injury, Degeneration, and Repair

11. Generation and Characterization of Mouse Models for Skeletal Disease

Gabrielle E. Foxa, Ye Liu, Lisa M. Turner, Alexander G. Robling, Tao Yang, and Bart O. Williams

12. Drill Hole Models to Investigate Bone Repair

Zhijun Li and Jill Helms

13. Generation and Experimental Outcomes of Closed Femoral Fracture in Mice

Joseph L. Roberts, Christopher W. Kinter, and Hicham Drissi

14. Mouse Models of Osteoarthritis: Surgical Model of Post-Traumatic Osteoarthritis Induced by Destabilization of the Medial Meniscus

Kirsty L. Culley, Purva Singh, Samantha Lessard, Mengying Wang, Brennan Rourke, Mary B. Goldring, and Miguel Otero

15. Immunostaining of Skeletal Tissues

Crystal Idleburg, Madelyn R. Lorenz, Elizabeth N. DeLassus, Erica L

• Format: Hardcover
• Language: English
• ISBN: 1071609882
• ISBN13: 9781071609880
• Release Date: September 2020
• Publisher: Humana
• Length: 302 Pages
• Weight: 1.60 lbs.
• Dimensions: 0.8" x 7.2" x 10.1"