Nitrogen Fixation: Methods and Protocols

Book Overview

Part I: Nitrogen Fixation

1. Historic Overview of Nitrogenase Research

Yilin Hu and Markus W. Ribbe

2. Mechanism of Mo-Dependent Nitrogenase

Zhi-Yong Yang, Karamatullah Danyal, and Lance C. Seefeldt

3. Assembly of Nitrogenase MoFe Protein

Chi-Chung Lee, Aaron W. Fay, Jared A. Wiig, Markus W. Ribbe, and Yilin Hu

4. Genomic Analysis of Nitrogen Fixation

Ina P. O'Carroll and Patricia C. Dos Santos

5. Enzymatic Systems with Homology to Nitrogenase

Markus J. Br?cker and J?rgen Moser

Part II: Genetic and Biochemical Methods

6. Molecular Biology and Genetic Engineering in Nitrogen Fixation

Patricia Dos Santos

7. Purification of Nitrogenase Proteins

Jared A. Wiig, Chi-Chung Lee, Aaron W. Fay, Yilin Hu, and Markus W. Ribbe

8. Assays of Nitrogenase Reaction Products

William E. Newton and Michael J. Dilworth

9. Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductase

Markus J. Br?cker and J?rgen Moser

Part III: Spectroscopic Methods

10. X-Ray Crystallography

Lauren E. Roth and F. Akif Tezcan

11. X-Ray Absorption Spectroscopy

Serena DeBeer

12. Small Angle X-Ray Scattering Spectroscopy

David W. Mulder and John W. Peters

13. Electron Paramagnetic Resonance Spectroscopy

Karamatullah Danyal, Zhi-Yong Yang, and Lance C. Seefeldt

14. Magnetic Circular Dichroism Spectroscopy

Brian J. Hales

15. M?ssbauer Spectroscopy

Boi Hanh Huynh

Part IV: Chemical Methods

16. Protocols for Cofactor Isoation of Nitrogenase

Aaron W. Fay, Chi-Chung Lee, Jared A. Wiig, Yilin Hu, and Markus W. Ribbe

17. Techniques for Functional and Structural Modeling of Nitrogenase

Patrick L. Holland

Part V: Theoretical Calculations

18. Nitrogenase Structure and Function Relationships by Density Functional Theory

Travis V. Harris and Robert K. Szilagyi

19. Modeling the MoFe Nitrogenase System with Broken Symmetry Density Functional Theory

Gregory M. Sandala and Louis Noodleman

• Format: Paperback
• Language: English
• ISBN: 1493958410
• ISBN13: 9781493958412
• Release Date: August 2016
• Publisher: Humana
• Length: 326 Pages
• Weight: 1.29 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"