Natural Killer Cells: Methods and Protocols

Book Overview

1. Tracking Effector and Memory NK Cells During MCMV Infection
Aimee M. Beaulieu and Joseph C. Sun

2. Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells
Alexander W. Kay, Dara M. Strauss-Albee, and Catherine A. Blish

3. Assessment of NK Cell Metabolism
Molly P. Keppel and Megan A. Cooper

4. Genotyping Single Nucleotide Polymorphisms and Copy Number Variability of the FCGRs Expressed on NK Cells
Amy K. Erbe, Wei Wang, Mikayla Gallenberger, Jacquelyn A. Hank, and Paul M. Sondel
5. Measurement of Average Telomere Length in Ex Vivo Expanded Natural Killer Cells by Fluorescence In Situ Hybridization (FISH)
Sourindra N. Maiti
6. In Vitro Assessment of Human Natural Killer Cell Migration and Invasion
Karin Tomin, Ronald H. Goldfarb, and Per Albertsson
7. Microfluidic-Based Live-Cell Analysis of NK-Cell Migration In Vitro
Saravanan Nandagopal, Francis Lin, and Sam K.P. Kung
8. Microwell-Based Live Cell Imaging of NK Cell Dynamics to Assess Heterogeneity in Motility and Cytotoxic Response
Bruno Vanherberghen, Thomas Frisk, Elin Forslund, Per E. Olofsson, Karolin Guldevall, and Bj?rn ?nfelt
9. Assessment of Natural Killer Cell Cytotoxicity Using Image Cytometry Method
Kelsey J. McCulley and Srinivas S. Somanchi
10. Analysis of Intracellular Ca2+ Mobilization in Human NK Cell Subsets by Flow Cytometry
Jakob Theorell and Yenan T. Bryceson
11. Using NK Cell Lipid Raft Fractionation to Understand the Role of Lipid Rafts in NK Cell Receptor SignalingEsther Serrano-Pertierra and Carlos L?pez-Larrea
12. High- and Super-Resolution Microscopy Imaging of the NK Cell Immunological Synapse
Emily M. Mace and Jordan S. Orange
13. The Planar Lipid Bilayer System Serves as a Reductionist Approach for Studying NK Cell Immunological Synapses and their Functions
Grant Bertolet and Dongfang Liu
14. Expansion of NK Cells Using Genetically Engineered K562 Feeder Cells
Minh-Trang Thi Phan, Seung-hwan Lee, Sang-Ki Kim, and Duck Cho

15. Ex-Vivo Expansion of Human NK Cells Using K562 Engineered to Express Membrane Bound IL21
Srinivas S. Somanchi and Dean A. Lee

16. Large-Scale Culture and Genetic Modification of Human Natural Killer Cells for Cellular TherapyNatalia Lapteva, Robin Parihar, Lisa A. Rollins, Adrian P. Gee, and Cliona M. Rooney
17. Gene Modification of Human Natural Killer Cells Using a Retroviral VectorJoshua N. Kellner, Conrad R. Cruz, Catherine M. Bollard, and Eric S. Yvon
18. Modification of Expanded NK Cells with Chimeric Antigen Receptor mRNA for Adoptive Cellular TherapyYaya Chu, Allyson Flower, and Mitchell S. Cairo
19. mRNA Transfection to Improve NK Cell Homing to TumorsEmily Levy, Mattias Carlsten, and Richard Childs
20. In Vitro Generation of Human NK Cells Expressing Chimeric Antigen Receptor through Differentiation of Gene-Modified Hematopoietic Stem CellsEmily Lowe, L

• Format: Hardcover
• Language: English
• ISBN: 1493936824
• ISBN13: 9781493936823
• Release Date: May 2016
• Publisher: Humana
• Length: 365 Pages
• Weight: 1.94 lbs.
• Dimensions: 0.9" x 7.0" x 10.0"