Mycobacterium Ulcerans: Methods and Protocols

Book Overview

Preface...Table of Contents...Contributing Authors...
Part I Mycobacterium ulcerans Disease and Host Pathogen Interaction
1. Overview: Mycobacterium ulcerans Disease (Buruli ulcer)Katharina R?ltgen and Gerd Pluschke
2. Immunohistochemistry: A Method to Analyze M.ulcerans Infected Skin TissueMarie-Th?r?se Ruf
3. Primary Isolation of Mycobacterium ulceransDorothy Yeboah-Manu, Adwoa Asante-Poku, and Samuel Yaw Aboagye
4. Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon ResonanceChristopher J. Day, Jessica Poole, Gerd Pluschke, and Michael P. Jennings
5. Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans CultureM?lanie Foulon, Estelle Marion, and Laurent Marsollier
6. DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCRCaroline J. Lavender and Janet A.M. Fyfe
7. Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulceransJanet A.M. Fyfe and Caroline J. Lavender
8. Detection of Mycobacterium ulcerans DNA using Real-Time PCR Janet A.M. Fyfe and Caroline J. Lavender
9. Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCRMarcus Beissner, Richard Odame Phillips, and Gisela Bretzel
10. Methods and Approaches for Buruli ulcer Surveillance in Africa: Lessons Learnt and Future DirectionsJoseph W. S. Timothy, Rachel L. Pullan, and Rie R. Yotsu

Part II Purification and Quantification of Myolactone
11. Overview: Mycolactone, The Macrolide Toxin of Mycobacterium ulceransKatharina R?ltgen and Gerd Pluschke
12. Conditions for Handling and Optimal Storage of MycolactoneJessica Z. Kubicek-Sutherland, Basil I. Swanson, and Harshini Mukundan
13. Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples Aline Rifflet, Caroline Demangel, and Laure Guenin-Mac?
14. Detection of Mycolactone by Thin Layer ChromatographyRichard Kwamla Amewu and Thomas Spangenberg
15. Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans MycolactoneLouisa Warryn
16. Biochemical and Biological Assays of Mycolactone-Mediated Inhibition of Sec61Sarah O'Keefe, Stephen High, and Caroline Demangel
Part III Drug Development against Mycobacterium ulcerans
17. Overview: Development of Drugs against Mycobacterium ulceransGerd Pluschke and Katharina R?ltgen
18. In Vitro Assessment of Drug Activity against Mycobacterium ulceransOliver D. Komm, Deepak V. Almeida, Paul J. Converse, and Eric L. Nuermberger
19. Drug Efficacy Testing in the Mouse Footpad Model of Buruli ulcerPaul J. Converse, Deepak V. Almeida, and Eric L. Nuermberger
20. Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulceransSangeeta Susan Thomas, Nitin Pal Kalia, and Kevin Pethe
21. Determination of Bioenergetic Parameters in Mycobacterium ulceransSangeeta Susan Thomas and Kevin Pethe
Subject Index List...

• Format: Paperback
• Language: English
• ISBN: 1071617818
• ISBN13: 9781071617816
• Release Date: October 2022
• Publisher: Humana
• Length: 232 Pages
• Weight: 0.95 lbs.
• Dimensions: 0.5" x 7.0" x 10.0"