Monoclonal antibodies preparation through production of hybridomas secreting specific antibodies against tetanus toxin. The hybridomas has a characteristic feature of both parent cells the ability to grow indefinitely from myeloma and the ability to produce the specific antibody from the B-cells. In this study the production of monoclonal antibodies against tetanus toxin which have a many advantages than the polyclonal antibodies(serum): 1- The monoclonal antibodies have a low tendency to produce hypersensitivity reaction that can caused by the polyclonal antibodies prepared in equine. 2-Monoclonal antibodies production need only for lab to production rather than polyclonal antibodies need farm to produce antitetanic serum in equine.
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