Molecular modelling and simulation of retroviral proteins and nanobiocomposites

By Sergey Shityakov and Sergey Shityakov

Doctoral Thesis / Dissertation from the year 2011 in the subject Biology - Micro- and Molecular Biology, grade: 1 (magna cum laude), University of W rzburg (Theodor-Boveri-Insitut f r Biowissenschaften), course: computer science, virology, biophysics, language: English, abstract: HIV-1 integrase has nuclear localization signals (NLS) which play a crucial role in nuclear import of viral preintegration complex (PIC). However, the detailed mechanisms of PIC formation and its nuclear transport are not known. I investigated the interaction of this viral protein HIV-1 integrase with proteins of the nuclear pore complex such as transportin-SR2 (Shityakov et al., 2010). I showed that the transportin-SR2 in nuclear import is required due to its interaction with the HIV-1 integrase. I analyzed key domain interaction, and hydrogen bond formation in transportin-SR2. In this thesis, I compared the transduction frequencies of PPT modified FV vectors with lentiviral vectors in nondividing and dividing alveolar basal epithelial cells from human adenocarcinoma (A549) by using molecular cloning, transfection and transduction techniques and several other methods. In contrast to lentiviral vectors, FV vectors were not able to efficiently transduce nondividing cell (Shityakov and Rethwilm, unpublished data). Despite the findings, which support the use of FV vectors as a safe and efficient alternative to lentiviral vectors, major limitation in terms of foamy-based retroviral vector gene transfer in quiescent cells still remains. In computational drug design I used molecular modelling methods such as lead expansion algorithm (Tripos(R)) to create a virtual library of compounds with different binding affinities to protease binding site. Further computational analyses revealed one unique compound with different protease binding ability from the initial hit and its role for possible new class of protease inhibitors is discussed (Shityakov and Dandekar, 2009). The phenomenon of an intercalat

• Format: Paperback
• Language: English
• ISBN: 3640933087
• ISBN13: 9783640933082
• Release Date: June 2011
• Publisher: Grin Verlag
• Length: 116 Pages
• Weight: 0.35 lbs.
• Dimensions: 0.3" x 5.8" x 8.3"