Molecular Chaperones: Methods and Protocols

Book Overview

1. Targeted Deletion of Hsf1, 2, and 4 Genes in Mice

Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, and Nahid F. Mivechi

2. The Role Of Heat Shock Factors in Stress-Induced Transcription

Yue Zhang, Shiuh-Dih Chou, Ayesha Murshid, Thomas L. Prince, Sheila Schreiner, Mary Ann Stevenson, and Stuart K. Calderwood

3. Hsp90 and Client Protein Maturation

Natalie Wayne, Parul Mishra, and Daniel N. Bolon

4. The Role of p23, Hop, Immunophilins, and Other Co-Chaperones in Regulating Hsp90 Function

Marc B. Cox and Jill L. Johnson

5. Detecting Hsp90 Phosphorylation

Mehdi Mollapour and Len Neckers

6. Role of Molecular Chaperones in Biogenesis of the Protein Kinome

Atin K. Mandal, Maria A. Theodoraki, Nadinath B. Nillegoda, and Avrom J. Caplan

7. Nucleotide Exchange Factors for Hsp70 Chaperones

Heike Rampelt, Matthias P. Mayer, and Bernd Bukau

8. Reconstitution of CHIP E3 Ubiquitin Ligase Activity

Hong Yu Ren, Cam Patterson, Douglas M. Cyr, and Meredith F.N. Rosser

9. Structure-Functions of HspB1 (Hsp27)

Andr?-Patrick Arrigo

10. Combined Lentiviral and RNAi Technologies for the Delivery and Permanently Silencing of the hsp25 Gene

Punit Kaur, Ganachari M. Nagaraja, Khalid Bin Abdulrahman, and Alexzander Asea

11. Quantification of HSP27 and HSP70 Molecular Chaperone Activities

Jessica Gobbo, Caroline Gaucher-Di-Stasio, St?phanie Weidmann, Jean Guzzo, and Carmen Garrido

12. Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA

H. Elyse Ireland and John H.H. Williams

13. Analysis of Heat Shock Protein Localization Using Flow Cytometry

Nina C. Dempsey, Francesca Leoni, Christine Hoyle, and John. H.H. Williams

14. Quantitation of Heat Shock Proteins in Clinical Samples Using Mass Spectrometry

Punit Kaur, Khalid Bin Abdulrahman, and Alexzander Asea

15. Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks

Yunchen Gong, Zhaolei Zhang, and Walid A. Houry

16. Hsp70: Anti-Apoptotic and Tumorigenic Protein

Anne-Laure R?role, Ga?tan Jego, and Carmen Garrido

17. Determination of Cell Survival or Death

Alexander E. Kabakov, Vladimir A. Kudryavtsev, and Vladimir L. Gabai

18. Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer

Francesco Cappello, Everly Conway de Macario, Giovanni Zummo, and Alberto J.L. Macario

19. Preparation of a Heat Shock Proteins 70-Based Vaccine from DC-Tumor Fusion Cells

Desheng Weng, Stuart K. Calderwood, and Jianlin Gong

20. Isolation of Heat Shock Protein Complexes

Fernando D. Cuello-Carri?n, Mariel A. Fanelli, Niubys Cayado-Guti?rrez, Gisela Castro, and Daniel R. Ciocca

21. Enhancing Antigen Cross-Presentation and T-Cell Priming by Complexing Protein Antigen to Recombinant Large Heat Shock Protein

Xiang-Yang Wang, Huanfa Yi, Xiaofei Yu, Damin Zuo, and John R. Subjeck

22. Investigating Receptors for Extracellular Heat Shock Proteins

Ayesha Murshid, Jimmy Theriault, Jianlin Gong, and Stuart K. Calderwood

23. Analysis of Cellular Migration Using a Two-Chamber Methodology

• Format: Paperback
• Language: English
• ISBN: 1493961705
• ISBN13: 9781493961702
• Release Date: August 2016
• Publisher: Humana
• Length: 321 Pages
• Weight: 1.30 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"