MicroRNAs in Development: Methods and Protocols

By: Dalmay, Tamas

Book Overview

1. In situ Detection of microRNAs in Animals

Dylan Sweetman

2. Detection of microRNAs in Plants by In situ Hybridization

?va V?rallyay and Zolt?n Havelda

3. Detecting Animal and Plant microRNAs by Northern Blotting

Sara L?pez-Gomoll?n

4. Profiling microRNAs by Real-time PCR

Nana Jacobsen, Ditte Andreasen, and Peter Mouritzen

5. Detection of Small RNAs and microRNAs using Deep Sequencing Technology

Ericka R. Havecker

6. Detection of microRNAs in Prostate Cancer Cells by microRNA Array

Xiaoqing Tang, Xiaohu Tang, Jozsef Gal, Natasha Kyprianou, Haining Zhu , and Guiliang Tang

7. microRNA Knock Down by Cholesterol Conjugated Antisense Oligos in Mouse Organ Culture

Sharon Kredo-Russo and Eran Hornstein

8. Protocols for Use of Homologous Recombination Gene-targeting to Produce microRNA Mutants in Drosophila

Ya-Wen Chen, Ruifen Weng, and Stephen M. Cohen

9. Engineering Elements for Gene Silencing: The Artificial microRNAs Technology

Pablo Andr?s Manavella and Ignacio Rubio-Somoza

10. Mimicry Technology: Suppressing Small RNA Activity in Plants

Ignacio Rubio-Somoza and Pablo Andr?s Manavella

11. Experimental Validation of MicroRNA Targets using a Luciferase Reporter System

Francisco E. Nicolas

12. Experimental Identification of microRNA Targets by Immunoprecipitation of Argonaute Protein Complexes

Michaela Beitzinger and Gunter Meister

13. Comprehensive Identification of miRNA Target Sites in Live Animals

Dimitrios G. Zisoulis , Gene W. Yeo, and Amy E. Pasquinelli

14. Target Validation of Plant microRNAs

C?sar Llave, Jos? Manuel Franco-Zorrilla, Roberto Solano, and Daniel Barajas

15. A High-throughput Sequencing-based Methodology to Identify All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes

Matthew W. Endres, Rebecca T. Cook, and Brian D. Gregory

• Format: Hardcover
• Language: English
• ISBN: 1617790826
• ISBN13: 9781617790829
• Release Date: March 2011
• Publisher: Humana Press
• Length: 210 Pages
• Weight: 1.35 lbs.
• Dimensions: 0.8" x 7.1" x 10.1"