Micrornas as Tools in Biopharmaceutical Production

By Niall Barron

Book Overview

Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.

• Format: Paperback
• Language: English
• ISBN: 9400795262
• ISBN13: 9789400795266
• Release Date: December 2014
• Publisher: Springer
• Length: 126 Pages
• Weight: 0.44 lbs.
• Dimensions: 0.3" x 6.1" x 9.2"