Microbial Steroids: Methods and Protocols

Book Overview

Preface...
Table of Contents...
Contributing Authors...

1. Steroid Bioconversions
Marina V. Donova

2. Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins
Ignacio Herr?iz

3. Genome Sequencing of Steroid Producing Bacteria with Illumina Technology
Victoria Y. Shtratnikova, Mikhail I. Schelkunov, and marina V. Donova

4. Genome Sequencing of Steroid Producing Bacteria using Ion Torrent Technology and a Reference Genome
Alberto Sola-Landa, Antonio Rodr?guez-Garc?a, Carlos Barreiro, and Rosario P?rez-Redondo

5. RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics
Antonio Rodr?guez-Garc?a, Alberto Sola-Landa, and Carlos Barreiro

6. Intra- and Extra-Cellular Proteome Analyses of Steroid-Producer Mycobacteria
Carlos Barreiro, Alejandro Morales, In?s V?zquez-Iglesias, and Alberto Sola-Landa

7. Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacteriumneoaurum NRRL B-3805
Jessica K. Loraine and Margaret C. M. Smith

8. Identification and Characterization of the genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas
Jos? M. Luengo and Elias R. Olivera

9. Selection of Biodegrading Phytosterol Strains
Mar?a-Ang?lica Mondaca, Maricel Vidal, Soledad Chamorro, and Gladys Vidal

10. Stigmasterol Removal by an Aerobic Treatment System
Soledad Chamorro, Claudia Xavier, V?ctor Hern?ndez, Jos? Becerra, and Gladys Vidal

11. Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3, 17-Dione
Rodrigo A. Mancilla, Roberto Pavez-D?az, and Andrea Mu?oz-Orrego

12. Β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates
Marco P.C. Marques and Pedro Fernandez

13. Bioconversion of Phytosterols into Androstenedione by Mycobacterium
Kjell D. Josefsen, Anna Nordborg, and H?vard Sletta

14. Scale-Up of Phytosterols Bioconversion into Androstenedione
Sonia Mart?nez-C?mara, Esther Bah?llo, Jos?-Luis Barredo, and Marta Rodr?guez-S?iz

15. Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331
Julia Garc?a-Fern?ndez, Igor Mart?nez, Lorena Fern?ndez-Cabez?n, Carmen Felpeto-Santero, Jos?-Luis Garc?a, and Beatriz Gal?n

16. Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1, 4-Diene-3, 17-Dione (ADD)
Surya Prakash and Abhay Bajaj

17. Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants
Sabrina Hoebenreich, Martin Spinck, and Nathalie Nett

18. Obtaining of 11α-Hydroxy and Rost-4-ene-3, 17-Dione from Natural Sterols
Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, Marina Donova

19. Steroid 11-Alfa-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus
Lidia Ortega-de los R?os, Jos? M. Luengo, and Jos? M. Fern?ndez-Ca??n

20. Biotransformation of DHEA into 7α, 15α-diOH-DHEA
Hui Li, Jin Sun, and Zhenghong Xu

21. Stabilization of Enzymes by Using Thermophiles
Ana-Luisa Ribeiro, Mercedes S?nchez, Aurelio Hidalgo, and Jos? Berenguer

22. Biocatalysis of Steroids with Mycobacterium Sp. in Aqueous and Organic Media
Carla C. C. R. de Carvalho and Pedro Fernandes

23. Downstream Process Synthesis for Microbial Steroids
Fabian B. Thygs and Juliane Merz

24. Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin Layer Chromatography (TLC)
Govinda Guevara, Juli?n Perera, and Juana-Mar?a Navarro-Llorens

• Format: Paperback
• Language: English
• ISBN: 1493984101
• ISBN13: 9781493984107
• Release Date: August 2018
• Publisher: Humana
• Length: 373 Pages
• Weight: 1.19 lbs.
• Dimensions: 0.8" x 6.1" x 9.2"