Planar Cell Polarity: Methods and Protocols

1. Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Locations

Daryl J.V. David, Melanie A. McGill, R.F. Andrew McKinley, and Tony J.C. Harris

2. Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell

Bertrand Jauffred and Yohanns Bellaiche

3. Protein-Protein Interaction Techniques: Dissect PCP Signaling in Xenopus

Yingqun Wang

4. Cuticle Refraction Microscopy (CRM): A Rapid and Simple Method for Imaging Drosophila Wing Topography, an Alternative Readout of Wing Planar Cell Polarity

David Neff, Justin Hogan, and Simon Collier

5. Analysis of Cell Shape and Polarity during Zebrafish Gastrulation

Douglas C. Weiser and David Kimelman

6. Analyzing Planar Cell Polarity during Zebrafish Gastrulation

Jason R. Jessen

7. Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements during Xenopus Gastrulation

Gun-Hwa Kim, Edmond Changkyun Park, and Jin-Kwan Han

8. Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling

Hyun-Shik Lee, Sergei Y. Sokol, Sally A. Moody, and Ira O. Daar

9. Gene Loss-of-Function and Live Imaging in Chick Embryos

Anne C. Rios, Christophe Marcelle, and Olivier Serralbo

10. Activation and Function of Small GTPases Rho, Rac, and Cdc42 during Gastrulation

Courtney Mezzacappa, Yuko Komiya, and Raymond Habas

11. Convergent Extension Analysis in Mouse Whole Embryo Culture

Sophie E. Pryor, Valentina Massa, Dawn Savery, Nicholas D.E. Greene, and Andrew J. Copp

12. Analysis of PCP Defects in Mammalian Eye Lens

Yuki Sugiyama and John W. McAvoy

13. Examining Planar Cell Polarity (PCP) in the Mammalian Cochlea

Helen May-Simera and Matthew W. Kelley

14. Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells

Lisa Fujimura and Masahiko Hatano

15. The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration

Kristi LaMonica and Laura Grabel

16. Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells

Mitsuharu Endo, Michiru Nishita, and Yasuhiro Minami

17. Regulation of Focal Adhesion Dynamics by Wnt5a Signaling

Shinji Matsumoto and Akira Kikuchi

18. The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity

Makoto Matsuyama and Akihiko Shimono

19. Assessing PCP in the Cochlea of Mammalian Ciliopathy Models

Daniel J. Jagger and Andrew Forge

20. Morphometric Analysis of Centrosome Position in Tissues

Hester Happ?, Emile de Heer, and Dorien J.M. Peters

• Format: Hardcover
• Language: English
• ISBN: 1617795097
• ISBN13: 9781617795091
• Release Date: January 2012
• Publisher: Humana
• Length: 262 Pages
• Weight: 1.45 lbs.
• Dimensions: 0.9" x 7.0" x 10.1"