Baculovirus and Insect Cell Expression Protocols

Book Overview

Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology(TM) series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.

• Format: Paperback
• Language: English
• ISBN: 1617376299
• ISBN13: 9781617376290
• Release Date: November 2010
• Publisher: Humana
• Length: 470 Pages
• Weight: 1.42 lbs.
• Dimensions: 1.0" x 6.0" x 9.0"