P53 Protocols

By Sumitra Deb

Book Overview

1. Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues

Marie P. Khoury, Virginie Marcel, Kenneth Fernandes, Alexandra Diot, David P. Lane and Jean-Christophe Bourdon

2. Detecting p53 Isoforms at Protein Level

Virginie Marcel, Marie P. Khoury, Kenneth Fernandes, Alexandra Diot, David P. Lane and Jean-Christophe Bourdon

3. Autophagy, Senescence and Apoptosis

Rachel W. Goehe, Molly L. Bristol, Eden N. Wilson, and David A. Gewirtz

4. p53 and Cell Cycle Effects after DNA Damage

Emir Senturk, James J. Manfredi

5. p53 Ubiquitination and Proteasomal Degradation

Ian Love, Dingding Shi, Steven R. Grossman

6. Identification of p53 in Mitochondria

Angelina V. Vaseva and Ute M. Moll

7. Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis

Sumitra Deb and Paul R. Graves

8. Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization

Qi Zhang, and Hua Lu

9. Determine the Effect of p53 on Chemosensitivity

Emir Senturk, James J. Manfredi

10. Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells

Mahesh Ramamoorthy, Catherine Vaughan, Sumitra Deb, Swati Palit Deb

11. Mutant p53 in Cell Adhesion and Motility

W. Andrew Yeudall, Katharine H. Wrighton, and Sumitra Deb

12. Use of the DNA Fiber Spreading Technique to Detect the Effects of Mutant p53 on DNA Replication

Rebecca A. Frum, Sumitra Deb and Swati Palit Deb

13. Generation of p53-deficient Induced Pluripotent Stem Cells (iPS) from Mouse Embryo Fibroblasts

Evguenia M. Alexandrova and Ute M. Moll

14. p53 Actions on MicroRNA Expression and Maturation Pathway

Hiroshi I. Suzuki and Kohei Miyazono

15. Isolation and Characterization of Murine Multipotent Lung Stem Cells

Venkat S Gadepalli, Catherine A Vaughan, Raj R. Rao

16. Generation of p53 Knock-down Cell Lines

Catherine Vaughan, Swati Palit Deb, Sumitra Deb

17. ChIP for Identification of p53 Responsive DNA Promoters

Jun-Ming Liao and Hua Lu

18. ChIP-on-chip to Identify Mutant p53 Targets

Frauke Goeman, Giulia Fontemaggi, and Giovanni Blandino

19. ChIP Sequencing to Identify p53 Targets

Catherine Vaughan, Brad Windle, Sumitra Deb

20. The p53-Mdm2 Loop: A Critical Juncture of Stress Response

Yaara Levav-Cohen, Zehavit Goldberg, Valentina Zuckerman, Sue Haupt, and Ygal Haupt

• Format: Paperback
• Language: English
• ISBN: 1493959336
• ISBN13: 9781493959334
• Release Date: August 2016
• Publisher: Humana
• Length: 238 Pages
• Weight: 0.98 lbs.
• Dimensions: 0.5" x 7.0" x 10.0"