Nitric Oxide Protocols (Methods in Molecular Biology)

By Michael A. Titheradge

This work provides both the experienced researcher and the newcomer with time-tested techniques to cover all aspects of nitric oxide synthase and metabolism. Topics range from the cloning and expression of the different isoforms of NO synthase to the role of NO in DNA damage and apoptosis and include indirect and direct measurements of NO production, measurement on NO synthase mRNA both in situ and in vitro, and Western blotting and immunohistochemical localization of different isoforms of NO synthase. A variety of alternative techniques are described to enable researchers to set up an assay using either the simplest equipment or the most expensive EPR machines.

• Format: Hardcover
• Language: English
• ISBN: 0896034704
• ISBN13: 9780896034709
• Release Date: January 1998
• Publisher: Humana Press
• Length: 324 Pages
• Weight: 1.55 lbs.
• Dimensions: 9.5" x 1.3" x 6.8"